Nucleolar and cytoplasmic localization of annexin V

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38 Citations (Scopus)

Abstract

The subcellular localization of annexin V in cultured human umbilical vein endothelial cells, epithelial cells and fibroblasts was examined. Indirect immunofluorescence and immunoblotting studies using affinity-purified anti-annexin V antibodies revealed that annexin V is located within the cytoplasm and nucleus of these cells. Further examination and direct binding studies showed that annexin V within the nucleus is associated with the nucleolus. These findings suggest that annexin V may play a role in a nucleolar function, such as ribosome assembly and transport.

Original languageEnglish
Pages (from-to)425-429
Number of pages5
JournalFEBS Letters
Volume314
Issue number3
DOIs
Publication statusPublished - 21 Dec 1992
Externally publishedYes

Keywords

  • Annexin V
  • Endothelial cell
  • Nucleolus

Cite this

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title = "Nucleolar and cytoplasmic localization of annexin V",
abstract = "The subcellular localization of annexin V in cultured human umbilical vein endothelial cells, epithelial cells and fibroblasts was examined. Indirect immunofluorescence and immunoblotting studies using affinity-purified anti-annexin V antibodies revealed that annexin V is located within the cytoplasm and nucleus of these cells. Further examination and direct binding studies showed that annexin V within the nucleus is associated with the nucleolus. These findings suggest that annexin V may play a role in a nucleolar function, such as ribosome assembly and transport.",
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Nucleolar and cytoplasmic localization of annexin V. / Sun, Jiuru; Salem, Hatem H.; Bird, Philip.

In: FEBS Letters, Vol. 314, No. 3, 21.12.1992, p. 425-429.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - Nucleolar and cytoplasmic localization of annexin V

AU - Sun, Jiuru

AU - Salem, Hatem H.

AU - Bird, Philip

PY - 1992/12/21

Y1 - 1992/12/21

N2 - The subcellular localization of annexin V in cultured human umbilical vein endothelial cells, epithelial cells and fibroblasts was examined. Indirect immunofluorescence and immunoblotting studies using affinity-purified anti-annexin V antibodies revealed that annexin V is located within the cytoplasm and nucleus of these cells. Further examination and direct binding studies showed that annexin V within the nucleus is associated with the nucleolus. These findings suggest that annexin V may play a role in a nucleolar function, such as ribosome assembly and transport.

AB - The subcellular localization of annexin V in cultured human umbilical vein endothelial cells, epithelial cells and fibroblasts was examined. Indirect immunofluorescence and immunoblotting studies using affinity-purified anti-annexin V antibodies revealed that annexin V is located within the cytoplasm and nucleus of these cells. Further examination and direct binding studies showed that annexin V within the nucleus is associated with the nucleolus. These findings suggest that annexin V may play a role in a nucleolar function, such as ribosome assembly and transport.

KW - Annexin V

KW - Endothelial cell

KW - Nucleolus

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EP - 429

JO - FEBS Letters

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