Nuclear receptor profiling of ovarian granulosa cell tumors

Maria Alexiadis, Natalie Eriksson, Stacey Jamieson, Melissa Davis, Ann Drummond, Simon Chu, Colin Clyne, George Muscat, Peter Fuller

Research output: Contribution to journalArticleResearchpeer-review

38 Citations (Scopus)

Abstract

Granulosa cell tumors of the ovary (GCT) represent 5 of malignant ovarian tumors. The adult form is defined by a mutation in the FOXL2 gene. GCT exhibit many of the features of normal proliferating granulosa cells. We have profiled the expression of the 48 human nuclear receptors (NR) by quantitative RT-PCR in a panel of GCT and in two GCT-derived cell lines, COV434 and KGN. The highest level of expression is seen for COUP-TF2 with abundant expression of PPARI?, SF-1, and TR-I?. Estrogen receptor (ER)-I? is the most abundant of the steroid receptors with relatively high expression also of AR, ER-I?, and PR. The concordance of expression for each NR across the tumors is remarkably high with same discordance between the cell lines and the tumors, particularly the COV434 line. No significant differences were observed with respect to tumor stage for NR expression. These findings provide a full profile of NR expression in GCT which will enable full characterization of their roles and potential as therapeutic targets.
Original languageEnglish
Pages (from-to)157 - 169
Number of pages13
JournalHormones and Cancer
Volume2
Issue number3
DOIs
Publication statusPublished - 2011

Keywords

  • Steroid receptors
  • Ovarian
  • Tumor
  • FOXL2

Cite this