Nuclear magnetic resonance spectroscopy of the circadian clock of cyanobacteria

Yong Gang Chang, Roger Tseng, Nai Wei Kuo, Andy Liwang

Research output: Contribution to journalArticleResearchpeer-review

2 Citations (Scopus)

Abstract

The most well-understood circadian clock at the level of molecular mechanisms is that of cyanobacteria. This overview is on how solution-state nuclear magnetic resonance (NMR) spectroscopy has contributed to this understanding. By exciting atomic spin-β nuclei in a strong magnetic field, NMR obtains information on their chemical environments, inter-nuclear distances, orientations, and motions. NMR protein samples are typically aqueous, often at near-physiological pH, ionic strength, and temperature. The level of information obtainable by NMR depends on the quality of the NMR sample, by which we mean the solubility and stability of proteins. Here, we use examples from our laboratory to illustrate the advantages and limitations of the technique.

Original languageEnglish
Pages (from-to)93-102
Number of pages10
JournalIntegrative and Comparative Biology
Volume53
Issue number1
DOIs
Publication statusPublished - 2013
Externally publishedYes

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