Nuclear factor of activated T (NFAT) cells activity within CD4+ T cells is influenced by activation status and tissue localisation

Nicola L. Harris, Victoria Watt, Sam MacLenachan, Sean Diehl, Benjamin J. Marsland, Mercedes Rincon, Graham Le Gros

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Nuclear factor of activated T (NFAT) cells is a family of transcription factors important for the regulation of cytokine expression by CD4+ T cells. Whilst a number of studies have examined NFAT activity of in vitro generated CD4+ T helper (Th)1 and Th2 cells, regulation of NFAT during in vivo immune responses has yet to be elucidated. We show that NFAT activity in CD4+ T cells peaked at early time-points in the draining mediastinal lymph node of mice infected with influenza A (Flu) or Nippostrongylus brasiliensis (Nb). In contrast, low NFAT transcriptional activity was detected in CD4+ T cells isolated from the lung of either Flu or Nb infected mice, despite a greater proportion of cytokine-producing cells being present at this site. These findings indicate that the activation status and tissue microenvironment of effector CD4+ T cells can determine their requirement for NFAT-mediated transcription.

Original languageEnglish
Pages (from-to)232-237
Number of pages6
JournalMicrobes and Infection
Volume8
Issue number1
DOIs
Publication statusPublished - 1 Jan 2006
Externally publishedYes

Keywords

  • CD4+ T cell
  • Influenza
  • Lung
  • Lymph node
  • Parasite

Cite this

Harris, Nicola L. ; Watt, Victoria ; MacLenachan, Sam ; Diehl, Sean ; Marsland, Benjamin J. ; Rincon, Mercedes ; Le Gros, Graham. / Nuclear factor of activated T (NFAT) cells activity within CD4+ T cells is influenced by activation status and tissue localisation. In: Microbes and Infection. 2006 ; Vol. 8, No. 1. pp. 232-237.
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abstract = "Nuclear factor of activated T (NFAT) cells is a family of transcription factors important for the regulation of cytokine expression by CD4+ T cells. Whilst a number of studies have examined NFAT activity of in vitro generated CD4+ T helper (Th)1 and Th2 cells, regulation of NFAT during in vivo immune responses has yet to be elucidated. We show that NFAT activity in CD4+ T cells peaked at early time-points in the draining mediastinal lymph node of mice infected with influenza A (Flu) or Nippostrongylus brasiliensis (Nb). In contrast, low NFAT transcriptional activity was detected in CD4+ T cells isolated from the lung of either Flu or Nb infected mice, despite a greater proportion of cytokine-producing cells being present at this site. These findings indicate that the activation status and tissue microenvironment of effector CD4+ T cells can determine their requirement for NFAT-mediated transcription.",
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Nuclear factor of activated T (NFAT) cells activity within CD4+ T cells is influenced by activation status and tissue localisation. / Harris, Nicola L.; Watt, Victoria; MacLenachan, Sam; Diehl, Sean; Marsland, Benjamin J.; Rincon, Mercedes; Le Gros, Graham.

In: Microbes and Infection, Vol. 8, No. 1, 01.01.2006, p. 232-237.

Research output: Contribution to journalArticleResearchpeer-review

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