TY - JOUR
T1 - Nox2-containing NADPH oxidase and xanthine oxidase are sources of superoxide in mouse trachea
AU - Westover, Alana Jasmine
AU - Harrison, Craig Bryan
AU - Selemidis, Stavros
PY - 2009
Y1 - 2009
N2 - 1. Superoxide anion plays an important role in host defense against invading pathogens, and in the inflammation that arises in lungs. The aim of the present study was to elucidate whether the two key candidate superoxide producing enzymes in mammalian cells, Nox2- containing NADPH oxidase and xanthine oxidase, are responsible for superoxide production in mouse trachea. 2. Superoxide production by the isolated trachea, as measured by L-012- dependent chemiluminescence, was markedly reduced by superoxide dismutase (300U/ml) and the xanthine oxidase inhibitor, allopurinol (100muM). Tracheas from Nox2(-/-) mice had significantly lower levels of superoxide than control mice. 3. These novel findings suggest that superoxide production by mouse trachea is attributed to both Nox2-containing NADPH oxidase and xanthine oxidase.
AB - 1. Superoxide anion plays an important role in host defense against invading pathogens, and in the inflammation that arises in lungs. The aim of the present study was to elucidate whether the two key candidate superoxide producing enzymes in mammalian cells, Nox2- containing NADPH oxidase and xanthine oxidase, are responsible for superoxide production in mouse trachea. 2. Superoxide production by the isolated trachea, as measured by L-012- dependent chemiluminescence, was markedly reduced by superoxide dismutase (300U/ml) and the xanthine oxidase inhibitor, allopurinol (100muM). Tracheas from Nox2(-/-) mice had significantly lower levels of superoxide than control mice. 3. These novel findings suggest that superoxide production by mouse trachea is attributed to both Nox2-containing NADPH oxidase and xanthine oxidase.
UR - http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=19076165
U2 - 10.1111/j.1440-1681.2008.05126.x
DO - 10.1111/j.1440-1681.2008.05126.x
M3 - Article
SN - 0305-1870
VL - 36
SP - 331
EP - 333
JO - Clinical and Experimental Pharmacology and Physiology
JF - Clinical and Experimental Pharmacology and Physiology
ER -