Novel strategy for microsphere-mediated DNA transfection

Jessica G. Borger, Juan Manuel Cardenas-Maestre, Rose Zamoyska, Rosario M. Sanchez-Martin

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A new approach for microsphere-mediated delivery of plasmid DNA has been developed and successfully evaluated. Basic molecular biology techniques were used to linearize and functionalize plasmid DNA by aminomodification, enabling efficient conjugation to carboxy-functionalized microspheres. A T cell hybridoma line was successfully transfected as determined by the efficient expression of a biologically relevant YFP fusion protein. Moreover, our data identified microsphere-mediated delivery of plasmid DNA as a noninvasive, nontoxic, and efficient gene delivery method with the potential to be applied to transfection-resistant, nondividing primary cells, including naïve T cells.

Original languageEnglish
Pages (from-to)1904-1908
Number of pages5
JournalBioconjugate Chemistry
Issue number10
Publication statusPublished - 19 Oct 2011
Externally publishedYes

Cite this

Borger, J. G., Cardenas-Maestre, J. M., Zamoyska, R., & Sanchez-Martin, R. M. (2011). Novel strategy for microsphere-mediated DNA transfection. Bioconjugate Chemistry, 22(10), 1904-1908.