TY - JOUR
T1 - Novel strategy for microsphere-mediated DNA transfection
AU - Borger, Jessica G.
AU - Cardenas-Maestre, Juan Manuel
AU - Zamoyska, Rose
AU - Sanchez-Martin, Rosario M.
PY - 2011/10/19
Y1 - 2011/10/19
N2 - A new approach for microsphere-mediated delivery of plasmid DNA has been developed and successfully evaluated. Basic molecular biology techniques were used to linearize and functionalize plasmid DNA by aminomodification, enabling efficient conjugation to carboxy-functionalized microspheres. A T cell hybridoma line was successfully transfected as determined by the efficient expression of a biologically relevant YFP fusion protein. Moreover, our data identified microsphere-mediated delivery of plasmid DNA as a noninvasive, nontoxic, and efficient gene delivery method with the potential to be applied to transfection-resistant, nondividing primary cells, including naïve T cells.
AB - A new approach for microsphere-mediated delivery of plasmid DNA has been developed and successfully evaluated. Basic molecular biology techniques were used to linearize and functionalize plasmid DNA by aminomodification, enabling efficient conjugation to carboxy-functionalized microspheres. A T cell hybridoma line was successfully transfected as determined by the efficient expression of a biologically relevant YFP fusion protein. Moreover, our data identified microsphere-mediated delivery of plasmid DNA as a noninvasive, nontoxic, and efficient gene delivery method with the potential to be applied to transfection-resistant, nondividing primary cells, including naïve T cells.
UR - http://www.scopus.com/inward/record.url?scp=80054793868&partnerID=8YFLogxK
U2 - 10.1021/bc200289n
DO - 10.1021/bc200289n
M3 - Article
AN - SCOPUS:80054793868
SN - 1043-1802
VL - 22
SP - 1904
EP - 1908
JO - Bioconjugate Chemistry
JF - Bioconjugate Chemistry
IS - 10
ER -