Novel small IncX3 plasmid carrying the blaKPC-2 gene in high-risk Klebsiella pneumoniae ST11/CG258

Bruna Fuga, Melina Lorraine Ferreira, Louise Teixeira Cerdeira, Paola Amaral de Campos, Vinícius Lopes Dias, Iara Rossi, Luiz Gustavo Machado, Nilton Lincopan, Paulo Pinto Gontijo-Filho, Rosineide Marques Ribas

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This study used whole-genome sequencing (WGS) and PFGE to analysis KPC-2-producing Klebsiella pneumoniae strains from clinical specimens collected in Brazilian hospitals. The study identifies the emergence of a novel small IncX3 plasmid (pKPB11), 12,757-bp in length, in a high-risk K. pneumoniae ST11/CG258 lineage, a successful clonal group in Brazil, carrying the blaKPC-2 gene on a non-Tn4401 genetic element (NTEKPC-Ic). Comparative analysis of the pKPB11 showed that this plasmid reduced its size, losing part of its conjugation apparatus. The pKPB11 was also compared to another strain sequenced in this study (KPC89) that had the hybrid IncX3-IncU plasmid (pKP89), of approximately 45 kb in length, similarly carrying the blaKPC-2 gene on NTEKPC-Ic. To the best of our knowledge, pKPB11 is the first example of small IncX3 plasmid found in a high-risk KPC-2-producing K. pneumoniae ST11/CG258.

Original languageEnglish
Article number114900
Number of pages4
JournalDiagnostic Microbiology and Infectious Disease
Issue number2
Publication statusPublished - Feb 2020
Externally publishedYes


  • CG258
  • IncX3
  • Klebsiella pneumoniae
  • KPC-2
  • NTE

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