Novel small IncX3 plasmid carrying the blaKPC-2 gene in high-risk Klebsiella pneumoniae ST11/CG258

Bruna Fuga; Melina Lorraine Ferreira; Louise Teixeira Cerdeira; Paola Amaral de Campos; Vinícius Lopes Dias; Iara Rossi; Luiz Gustavo Machado; Nilton Lincopan; Paulo Pinto Gontijo-Filho; Rosineide Marques Ribas

doi:10.1016/j.diagmicrobio.2019.114900

Novel small IncX3 plasmid carrying the bla_KPC-2 gene in high-risk Klebsiella pneumoniae ST11/CG258

Bruna Fuga, Melina Lorraine Ferreira, Louise Teixeira Cerdeira, Paola Amaral de Campos, Vinícius Lopes Dias, Iara Rossi, Luiz Gustavo Machado, Nilton Lincopan, Paulo Pinto Gontijo-Filho, Rosineide Marques Ribas

Research output: Contribution to journal › Article › Research › peer-review

9 Citations (Scopus)

Abstract

This study used whole-genome sequencing (WGS) and PFGE to analysis KPC-2-producing Klebsiella pneumoniae strains from clinical specimens collected in Brazilian hospitals. The study identifies the emergence of a novel small IncX3 plasmid (pKPB11), 12,757-bp in length, in a high-risk K. pneumoniae ST11/CG258 lineage, a successful clonal group in Brazil, carrying the bla_KPC-2 gene on a non-Tn4401 genetic element (NTE_KPC-Ic). Comparative analysis of the pKPB11 showed that this plasmid reduced its size, losing part of its conjugation apparatus. The pKPB11 was also compared to another strain sequenced in this study (KPC89) that had the hybrid IncX3-IncU plasmid (pKP89), of approximately 45 kb in length, similarly carrying the bla_KPC-2 gene on NTE_KPC-Ic. To the best of our knowledge, pKPB11 is the first example of small IncX3 plasmid found in a high-risk KPC-2-producing K. pneumoniae ST11/CG258.

Original language	English
Article number	114900
Number of pages	4
Journal	Diagnostic Microbiology and Infectious Disease
Volume	96
Issue number	2
DOIs	https://doi.org/10.1016/j.diagmicrobio.2019.114900
Publication status	Published - Feb 2020
Externally published	Yes

Keywords

CG258
IncX3
Klebsiella pneumoniae
KPC-2
NTE

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.diagmicrobio.2019.114900

Cite this

Fuga, B., Ferreira, M. L., Cerdeira, L. T., de Campos, P. A., Dias, V. L., Rossi, I., Machado, L. G., Lincopan, N., Gontijo-Filho, P. P., & Ribas, R. M. (2020). Novel small IncX3 plasmid carrying the bla_KPC-2 gene in high-risk Klebsiella pneumoniae ST11/CG258. Diagnostic Microbiology and Infectious Disease, 96(2), Article 114900. https://doi.org/10.1016/j.diagmicrobio.2019.114900

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abstract = "This study used whole-genome sequencing (WGS) and PFGE to analysis KPC-2-producing Klebsiella pneumoniae strains from clinical specimens collected in Brazilian hospitals. The study identifies the emergence of a novel small IncX3 plasmid (pKPB11), 12,757-bp in length, in a high-risk K. pneumoniae ST11/CG258 lineage, a successful clonal group in Brazil, carrying the blaKPC-2 gene on a non-Tn4401 genetic element (NTEKPC-Ic). Comparative analysis of the pKPB11 showed that this plasmid reduced its size, losing part of its conjugation apparatus. The pKPB11 was also compared to another strain sequenced in this study (KPC89) that had the hybrid IncX3-IncU plasmid (pKP89), of approximately 45 kb in length, similarly carrying the blaKPC-2 gene on NTEKPC-Ic. To the best of our knowledge, pKPB11 is the first example of small IncX3 plasmid found in a high-risk KPC-2-producing K. pneumoniae ST11/CG258.",

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AU - de Campos, Paola Amaral

AU - Dias, Vinícius Lopes

AU - Rossi, Iara

AU - Machado, Luiz Gustavo

AU - Lincopan, Nilton

AU - Gontijo-Filho, Paulo Pinto

AU - Ribas, Rosineide Marques

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