Abstract
A number of studies require the determination of the genetic sex of mouse embryos before sexual differentiation and/or of mutant mice that display partial or complete sex reversal. The majority of current methods for sexing by PCR involve multiplexing of 2 primer pairs. We have developed a novel sexing PCR using a single primer pair that amplifies fragments from the X and the Y chromosome with a clear size difference between the respective amplicons. This assay provides a rapid and reliable method to identify the genetic sex of mice across different mouse strains.
| Original language | English |
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| Pages (from-to) | 207 - 211 |
| Number of pages | 5 |
| Journal | Sexual Development |
| Volume | 7 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2013 |