Novel lipase purification methods - a review of the latest developments

Chun-Hong Tan, Pau Loke Show, Chien Wei Ooi, Eng-Poh Ng, John Chi-Wei Lan, Tau Chuan Ling

    Research output: Contribution to journalShort ReviewOtherpeer-review

    28 Citations (Scopus)

    Abstract

    Microbial lipases are popular biocatalysts due to their ability to catalyse diverse reactions such as hydrolysis, esterification, and acidolysis. Lipases function efficiently on various substrates in aqueous and non-aqueous media. Lipases are chemo-, regio-, and enantio-specific, and are useful in various industries, including those manufacturing food, detergents, and pharmaceuticals. A large number of lipases from fungal and bacterial sources have been isolated and purified to homogeneity. This success is attributed to the development of both conventional and novel purification techniques. This review highlights the use of these techniques in lipase purification, including conventional techniques such as: (i) ammonium sulphate fractionation; (ii) ion-exchange; (iii) gel filtration and affinity chromatography; as well as novel techniques such as (iv) reverse micellar system; (v) membrane processes; (vi) immunopurification; (vi) aqueous two-phase system; and (vii) aqueous two-phase floatation. A summary of the purification schemes for various bacterial and fungal lipases are also provided.
    Original languageEnglish
    Pages (from-to)31 - 44
    Number of pages14
    JournalBiotechnology Journal
    Issue number1
    DOIs
    Publication statusPublished - 2015

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