Novel cell culture device enabling three-dimensional cell growth and improved cell function

Maria Bokhari, Ross J. Carnachan, Neil R. Cameron, Stefan A. Przyborski

Research output: Contribution to journalArticleResearchpeer-review

103 Citations (Scopus)

Abstract

A better understanding of cell biology and cell-cell interactions requires three-dimensional (3-D) culture systems that more closely represent the natural structure and function of tissues in vivo. Here, we present a novel device that provides an environment for routine 3-D cell growth in vitro. We have developed a thin membrane of polystyrene scaffold with a well defined and uniform porous architecture and have adapted this material for cell culture applications. We have exemplified the application of this technology by growing HepG2 liver cells on 2- and 3-D substrates. The performance of HepG2 cells grown on scaffolds was significantly enhanced compared to functional activity of cells grown on 2-D plastic. The incorporation of thin membranes of porous polystyrene to create a novel device has been successfully demonstrated as a new 3-D cell growth technology for routine use in cell culture.

Original languageEnglish
Pages (from-to)1095-1100
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume354
Issue number4
DOIs
Publication statusPublished - 23 Mar 2007
Externally publishedYes

Keywords

  • 3-D cell growth
  • Cell culture
  • Device
  • HepG2 cells
  • Liver
  • Polymer scaffold

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