Not always an innocent bystander: the impact of stabilised phosphopantetheine moieties when studying nonribosomal peptide biosynthesis

Y. T.Candace Ho (Leading Author), Joe A. Kaczmarski, Julien Tailhades, Thierry Izoré, David L. Steer, Ralf B. Schittenhelm, Manuela Tosin, Colin J. Jackson, Max J. Cryle (Leading Author)

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Nonribosomal peptide synthetases produce many important peptide natural products and are centred around carrier proteins (CPs) that deliver intermediates to various catalytic domains. We show that the replacement of CP substrate thioesters by stabilised ester analogues leads to active condensation domain complexes, whereas amide stabilisation generates non-functional complexes.

Original languageEnglish
Pages (from-to)8234-8237
Number of pages4
JournalChemical Communications
Volume59
Issue number53
DOIs
Publication statusPublished - 29 Jun 2023

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