Normalization of affymetrix miRNA microarrays for the analysis of cancer samples

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Abstract

microRNA (miRNA) microarray normalization is a critical step for the identification of truly differentially expressed miRNAs. This is particularly important when dealing with cancer samples that have a global miRNA decrease. In this chapter, we provide a simple step-by-step procedure that can be used to normalize Affymetrix miRNA microarrays, relying on robust normal-exponential background correction with cyclic loess normalization.

Original languageEnglish
Title of host publicationMicroarray Data Analysis
Subtitle of host publicationMethods and Applications
EditorsPietro Hiram Guzzi
PublisherHumana Press
Pages1-10
Number of pages10
Volume1375
Edition2nd
DOIs
Publication statusPublished - 1 May 2016

Publication series

NameMethods in Molecular Biology
PublisherSpringer
Volume1375
ISSN (Print)10643745

Keywords

  • Affymetrix
  • Cancer samples
  • MicroRNA
  • MiRNA microarray
  • Normalization

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