Normal survival and in-vitro development after cryopreservation of zona-drilled embryos in mice

Herman T. Depyper, John C. Carroll, Dirk Vandekerckhove, Colin D. Matthews

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10 Citations (Scopus)


The Importance of the zona pellucida on survival after freezing and thawing was Invesligated. Zona-drilled and zona Intact mouse embryos were fertilized in vitro, cultured to the 2, 4 and 8-cell stages and frozen using conventional methods. Zona drilling did not affect the survival or development of frozen embryos to the blastocyst stage in vitro. We conclude that partial damage to the zona pellucida during micromanipulation procedures Ls compatible with rates of survival and development which are not different to those observed In zona-Intact control embryos.

Original languageEnglish
Pages (from-to)432-435
Number of pages4
JournalHuman Reproduction
Issue number3
Publication statusPublished - 1 Jan 1991
Externally publishedYes


  • Cryopreservation
  • Embryos
  • Zona drilling

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