TY - JOUR
T1 - Non-viral delivery systems of DNA into stem cells
T2 - Promising and multifarious actions for regenerative medicine
AU - Morshed, Mahboob
AU - Hasan, Anwarul
AU - Sharifi, Majid
AU - Nejadi Babadaei, Mohammad Mahdi
AU - Bloukh, Samir Haj
AU - Islam, Mohammad Ariful
AU - Chowdhury, Ezharul Hoque
AU - Falahati, Mojtaba
PY - 2020/12
Y1 - 2020/12
N2 - Regenerative medicine has an exciting prospective to bring preventive and therapeutic benefits to a myriad of diseases. Different biological factors such as growth factors, therapeutic proteins, antibodies, plasmid DNA (pDNA), and some kinds of RNA can have an effect on stem cells (SCs) differentiation and underlying tissue regeneration. To understand the therapeutic perspectives of these modalities into SCs, they need to be delivered effectively into the targeted site. Since, the genes transfer via viral vectors will obstruct ultimate clinical translation and gene transfer based on naked pDNA is miserably incompetent, the improvement of non-viral gene delivery systems (GDSs) has been received a great deal of interest in tissue regeneration studies. Considering low immunogenicity and no threat of diffusion of communicable diseases with little construction rate, non-viral delivery systems have received considerable attention to deliver genes into SCs for tissue regeneration. In this review, we discussed several proteins for delivery of their genes and expression into cells for cell growth and differentiation into blood, bone, cardiac muscle, skeletal muscle, brain tissue, and so on. Also, some methods are discussed for potential delivery of growth factors where their manipulation and other signaling cascades in mesenchymal SCs (MSCs) are occurred for potential tissue regeneration.
AB - Regenerative medicine has an exciting prospective to bring preventive and therapeutic benefits to a myriad of diseases. Different biological factors such as growth factors, therapeutic proteins, antibodies, plasmid DNA (pDNA), and some kinds of RNA can have an effect on stem cells (SCs) differentiation and underlying tissue regeneration. To understand the therapeutic perspectives of these modalities into SCs, they need to be delivered effectively into the targeted site. Since, the genes transfer via viral vectors will obstruct ultimate clinical translation and gene transfer based on naked pDNA is miserably incompetent, the improvement of non-viral gene delivery systems (GDSs) has been received a great deal of interest in tissue regeneration studies. Considering low immunogenicity and no threat of diffusion of communicable diseases with little construction rate, non-viral delivery systems have received considerable attention to deliver genes into SCs for tissue regeneration. In this review, we discussed several proteins for delivery of their genes and expression into cells for cell growth and differentiation into blood, bone, cardiac muscle, skeletal muscle, brain tissue, and so on. Also, some methods are discussed for potential delivery of growth factors where their manipulation and other signaling cascades in mesenchymal SCs (MSCs) are occurred for potential tissue regeneration.
KW - Gene delivery
KW - Non-viral delivery
KW - Stem cell therapy
KW - Tissue regeneration
UR - http://www.scopus.com/inward/record.url?scp=85089467331&partnerID=8YFLogxK
U2 - 10.1016/j.jddst.2020.101861
DO - 10.1016/j.jddst.2020.101861
M3 - Review Article
AN - SCOPUS:85089467331
SN - 1773-2247
VL - 60
JO - Journal of Drug Delivery Science and Technology
JF - Journal of Drug Delivery Science and Technology
M1 - 101861
ER -
