Non-invasive assessment of the efficacy of new therapeutics for intestinal pathologies using serial endoscopic imaging of live mice

Matthias Ernst, Adele Preaudet, Tracy L Putoczki

Research output: Contribution to journalArticleOtherpeer-review

5 Citations (Scopus)

Abstract

Animal models of inflammatory bowel disease (IBD) and colorectal cancer (CRC) have provided significant insight into the cell intrinsic and extrinsic mechanisms that contribute to the onset and progression of intestinal diseases. The identification of new molecules that promote these pathologies has led to a flurry of activity focused on the development of potential new therapies to inhibit their function. As a result, various preclinical mouse models with an intact immune system and stromal microenvironment are now heavily used. Here we describe three experimental protocols to test the efficacy of new therapeutics in pre-clinical models of (1) acute mucosal damage, (2) chronic colitis and/or colitis-associated colon cancer, and (3) sporadic colorectal cancer. We also outline procedures for serial endoscopic examination that can be used to document the therapeutic response of an individual tumor and to monitor the health of individual mice. These protocols provide complementary experimental platforms to test the effectiveness of therapeutic compounds shown to be well tolerated by mice.

Original languageEnglish
Article numbere52383
JournalJournal of Visualized Experiments
Volume2015
Issue number97
DOIs
Publication statusPublished - 10 Mar 2015
Externally publishedYes

Keywords

  • Cancer
  • Colitis
  • Colon
  • Endoscopy
  • Issue 97
  • Medicine
  • Mucosa
  • Therapy

Cite this