Non-invasive airway health measurement using synchrotron X-ray microscopy of high refractive index glass microbeads

Martin Donnelley, Kaye Morgan, Nigel R Farrow, Karen Siu, David Parsons

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Cystic fibrosis (CF) is caused by a gene defect that compromises the ability of the mucociliary transit (MCT) system to clear the airways of debris and pathogens. To directly characterise airway health and the effects of treatments we have developed a synchrotron X-ray microscopy method that non-invasively measures the local rate and patterns of MCT behaviour. Although the nasal airways of CF mice exhibit the CF pathophysiology, there is evidence that nasal MCT is not altered in CF mice1. The aim of this experiment was to determine if our non-invasive local airway health assessment method could identify differences in nasal MCT rate between normal and CF mice, information that is potentially lost in bulk MCT measurements. Experiments were performed on the BL20XU beamline at the SPring-8 Synchrotron in Japan. Mice were anaesthetized, a small quantity of micron-sized marker particles were delivered to the nose, and images of the nasal airways were acquired for 15 minutes. The nasal airways were treated with hypertonic saline or mannitol to increase surface hydration and MCT. Custom software was used to locate and track particles and calculate individual and bulk MCT rates. No statistically significant differences in MCT rate were found between normal and CF mouse nasal airways or between treatments. However, we hope that the improved sensitivity provided by this technique will accelerate the ability to identify useful CF lung disease-modifying interventions in small animal models, and enhance the development and efficacy of proposed new therapies.

Original languageEnglish
Title of host publicationXRM 2014
Subtitle of host publicationProceedings of the 12th International Conference on X-Ray Microscopy
EditorsMartin D. de Jonge, David J. Paterson, Christopher G. Ryan
PublisherAmerican Institute of Physics
Number of pages4
ISBN (Electronic)9780735413436
Publication statusPublished - 28 Jan 2016
EventInternational Conference on X-ray Microscopy (XRM) 2014 - Melbourne Convention and Exhibition Centre, Melbourne, Australia
Duration: 26 Oct 201431 Oct 2014
Conference number: 12th


ConferenceInternational Conference on X-ray Microscopy (XRM) 2014
Abbreviated titleXRM 2014
Internet address

Cite this

Donnelley, M., Morgan, K., Farrow, N. R., Siu, K., & Parsons, D. (2016). Non-invasive airway health measurement using synchrotron X-ray microscopy of high refractive index glass microbeads. In M. D. de Jonge, D. J. Paterson, & C. G. Ryan (Eds.), XRM 2014: Proceedings of the 12th International Conference on X-Ray Microscopy (Vol. 1696). [020011] American Institute of Physics.