TY - JOUR
T1 - Non-contact current transfer induces the formation and improves the X-ray diffraction quality of protein crystals
AU - Boltsis, Ilias
AU - Lagoumintzis, George
AU - Chatzileontiadou, Demetra S.M.
AU - Giastas, Petros
AU - Tzartos, Socrates J.
AU - Leonidas, Demetres D.
AU - Poulas, Konstantinos
PY - 2014/9/3
Y1 - 2014/9/3
N2 - The effect of non-contact current transfer on the crystallization of three model proteins (hen egg white lysozyme, bovine pancreatic ribonuclease A, and human recombinant insulin) was investigated using a prototype ion-generator device. The device can produce an equivalent of microcurrent that can be transmitted in a contactless manner by spraying negatively charged ions and using available air gases (O2) as a transfer medium. From a crystallographic perspective, non-contact current transfer technology clearly enhanced the crystal formation process of these three proteins, as evidenced by the high crystallization speed and crystal quality. The quality and X-ray diffraction patterns of lysozyme crystals grown in the presence and absence of the devices microcurrent were analyzed. Our results indicate a significant positive effect on the quality of the X-ray diffraction pattern after non-contact current transfer, highlighting the value of this technique in protein crystallization.
AB - The effect of non-contact current transfer on the crystallization of three model proteins (hen egg white lysozyme, bovine pancreatic ribonuclease A, and human recombinant insulin) was investigated using a prototype ion-generator device. The device can produce an equivalent of microcurrent that can be transmitted in a contactless manner by spraying negatively charged ions and using available air gases (O2) as a transfer medium. From a crystallographic perspective, non-contact current transfer technology clearly enhanced the crystal formation process of these three proteins, as evidenced by the high crystallization speed and crystal quality. The quality and X-ray diffraction patterns of lysozyme crystals grown in the presence and absence of the devices microcurrent were analyzed. Our results indicate a significant positive effect on the quality of the X-ray diffraction pattern after non-contact current transfer, highlighting the value of this technique in protein crystallization.
UR - http://www.scopus.com/inward/record.url?scp=84906879082&partnerID=8YFLogxK
U2 - 10.1021/cg5004098
DO - 10.1021/cg5004098
M3 - Article
AN - SCOPUS:84906879082
VL - 14
SP - 4347
EP - 4354
JO - Crystal Growth and Design
JF - Crystal Growth and Design
SN - 1528-7483
IS - 9
ER -