Nogo receptor 1 regulates Caspr distribution at axo-glial units in the central nervous system

Jae Young Lee, Min Joung Kim, Lijun Li, Alexander A. Velumian, Pei Mun Aui, Michael G. Fehlings, Steven Petratos

Research output: Contribution to journalArticleResearchpeer-review

3 Citations (Scopus)

Abstract

Axo-glial units are highly organised microstructures propagating saltatory conduction and are disrupted during multiple sclerosis (MS). Nogo receptor 1 (NgR1) has been suggested to govern axonal damage during the progression of disease in the MS-like mouse model, experimental autoimmune encephalomyelitis (EAE). Here we have identified that adult ngr1 -/- mice, previously used in EAE and spinal cord injury experiments, display elongated paranodes, and nodes of Ranvier. Unstructured paranodal regions in ngr1 -/- mice are matched with more distributed expression pattern of Caspr. Compound action potentials of optic nerves and spinal cords from naïve ngr1 -/- mice are delayed and reduced. Molecular interaction studies revealed enhanced Caspr cleavage. Our data suggest that NgR1 may regulate axo-myelin ultrastructure through Caspr-mediated adhesion, regulating the electrophysiological signature of myelinated axons of central nervous system (CNS).

Original languageEnglish
Article number8958
Number of pages13
JournalScientific Reports
Volume7
Issue number1
DOIs
Publication statusPublished - 1 Dec 2017

Cite this

Lee, Jae Young ; Kim, Min Joung ; Li, Lijun ; Velumian, Alexander A. ; Aui, Pei Mun ; Fehlings, Michael G. ; Petratos, Steven. / Nogo receptor 1 regulates Caspr distribution at axo-glial units in the central nervous system. In: Scientific Reports. 2017 ; Vol. 7, No. 1.
@article{0e652e2a99114288bf4509c28055d54f,
title = "Nogo receptor 1 regulates Caspr distribution at axo-glial units in the central nervous system",
abstract = "Axo-glial units are highly organised microstructures propagating saltatory conduction and are disrupted during multiple sclerosis (MS). Nogo receptor 1 (NgR1) has been suggested to govern axonal damage during the progression of disease in the MS-like mouse model, experimental autoimmune encephalomyelitis (EAE). Here we have identified that adult ngr1 -/- mice, previously used in EAE and spinal cord injury experiments, display elongated paranodes, and nodes of Ranvier. Unstructured paranodal regions in ngr1 -/- mice are matched with more distributed expression pattern of Caspr. Compound action potentials of optic nerves and spinal cords from na{\"i}ve ngr1 -/- mice are delayed and reduced. Molecular interaction studies revealed enhanced Caspr cleavage. Our data suggest that NgR1 may regulate axo-myelin ultrastructure through Caspr-mediated adhesion, regulating the electrophysiological signature of myelinated axons of central nervous system (CNS).",
author = "Lee, {Jae Young} and Kim, {Min Joung} and Lijun Li and Velumian, {Alexander A.} and Aui, {Pei Mun} and Fehlings, {Michael G.} and Steven Petratos",
year = "2017",
month = "12",
day = "1",
doi = "10.1038/s41598-017-09405-9",
language = "English",
volume = "7",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",

}

Nogo receptor 1 regulates Caspr distribution at axo-glial units in the central nervous system. / Lee, Jae Young; Kim, Min Joung; Li, Lijun; Velumian, Alexander A.; Aui, Pei Mun; Fehlings, Michael G.; Petratos, Steven.

In: Scientific Reports, Vol. 7, No. 1, 8958, 01.12.2017.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Nogo receptor 1 regulates Caspr distribution at axo-glial units in the central nervous system

AU - Lee, Jae Young

AU - Kim, Min Joung

AU - Li, Lijun

AU - Velumian, Alexander A.

AU - Aui, Pei Mun

AU - Fehlings, Michael G.

AU - Petratos, Steven

PY - 2017/12/1

Y1 - 2017/12/1

N2 - Axo-glial units are highly organised microstructures propagating saltatory conduction and are disrupted during multiple sclerosis (MS). Nogo receptor 1 (NgR1) has been suggested to govern axonal damage during the progression of disease in the MS-like mouse model, experimental autoimmune encephalomyelitis (EAE). Here we have identified that adult ngr1 -/- mice, previously used in EAE and spinal cord injury experiments, display elongated paranodes, and nodes of Ranvier. Unstructured paranodal regions in ngr1 -/- mice are matched with more distributed expression pattern of Caspr. Compound action potentials of optic nerves and spinal cords from naïve ngr1 -/- mice are delayed and reduced. Molecular interaction studies revealed enhanced Caspr cleavage. Our data suggest that NgR1 may regulate axo-myelin ultrastructure through Caspr-mediated adhesion, regulating the electrophysiological signature of myelinated axons of central nervous system (CNS).

AB - Axo-glial units are highly organised microstructures propagating saltatory conduction and are disrupted during multiple sclerosis (MS). Nogo receptor 1 (NgR1) has been suggested to govern axonal damage during the progression of disease in the MS-like mouse model, experimental autoimmune encephalomyelitis (EAE). Here we have identified that adult ngr1 -/- mice, previously used in EAE and spinal cord injury experiments, display elongated paranodes, and nodes of Ranvier. Unstructured paranodal regions in ngr1 -/- mice are matched with more distributed expression pattern of Caspr. Compound action potentials of optic nerves and spinal cords from naïve ngr1 -/- mice are delayed and reduced. Molecular interaction studies revealed enhanced Caspr cleavage. Our data suggest that NgR1 may regulate axo-myelin ultrastructure through Caspr-mediated adhesion, regulating the electrophysiological signature of myelinated axons of central nervous system (CNS).

UR - http://www.scopus.com/inward/record.url?scp=85029414868&partnerID=8YFLogxK

U2 - 10.1038/s41598-017-09405-9

DO - 10.1038/s41598-017-09405-9

M3 - Article

VL - 7

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

M1 - 8958

ER -