NMR study of Met-1 human Angiogenin: 1H, 13C, 15N backbone and side-chain resonance assignment

Aikaterini C. Tsika, Demetra S.M. Chatzileontiadou, Demetres D. Leonidas, Georgios A. Spyroulias

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Here, we report the high yield expression and preliminary structural analysis via solution hetero-nuclear NMR spectroscopy of the recombinant Met-1 human Angiogenin. The analysis reveals a well folded as well as, a monomeric polypeptide. Τhe sequence-specific assignment of its 1H, 15N and 13C resonances at high percentage was obtained. Also, using TALOS+ its secondary structure elements were determined.

Original languageEnglish
Pages (from-to)379-383
Number of pages5
JournalBiomolecular NMR Assignments
Issue number2
Publication statusPublished - 1 Oct 2016
Externally publishedYes


  • Angiogenesis
  • Angiogenin
  • NMR spectroscopy
  • Recombinant protein expression
  • RNase superfamily

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