NMR study of Met-1 human Angiogenin: 1H, 13C, 15N backbone and side-chain resonance assignment

Aikaterini C. Tsika, Demetra S.M. Chatzileontiadou, Demetres D. Leonidas, Georgios A. Spyroulias

Research output: Contribution to journalArticleResearchpeer-review

3 Citations (Scopus)

Abstract

Here, we report the high yield expression and preliminary structural analysis via solution hetero-nuclear NMR spectroscopy of the recombinant Met-1 human Angiogenin. The analysis reveals a well folded as well as, a monomeric polypeptide. Τhe sequence-specific assignment of its 1H, 15N and 13C resonances at high percentage was obtained. Also, using TALOS+ its secondary structure elements were determined.

Original languageEnglish
Pages (from-to)379-383
Number of pages5
JournalBiomolecular NMR Assignments
Volume10
Issue number2
DOIs
Publication statusPublished - 1 Oct 2016
Externally publishedYes

Keywords

  • Angiogenesis
  • Angiogenin
  • NMR spectroscopy
  • Recombinant protein expression
  • RNase superfamily

Cite this