Abstract
Here, we report the high yield expression and preliminary structural analysis via solution hetero-nuclear NMR spectroscopy of the recombinant Met-1 human Angiogenin. The analysis reveals a well folded as well as, a monomeric polypeptide. Τhe sequence-specific assignment of its 1H, 15N and 13C resonances at high percentage was obtained. Also, using TALOS+ its secondary structure elements were determined.
| Original language | English |
|---|---|
| Pages (from-to) | 379-383 |
| Number of pages | 5 |
| Journal | Biomolecular NMR Assignments |
| Volume | 10 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 1 Oct 2016 |
| Externally published | Yes |
Keywords
- Angiogenesis
- Angiogenin
- NMR spectroscopy
- Recombinant protein expression
- RNase superfamily
Cite this
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NMR study of Met-1 human Angiogenin : 1H, 13C, 15N backbone and side-chain resonance assignment. / Tsika, Aikaterini C.; Chatzileontiadou, Demetra S.M.; Leonidas, Demetres D.; Spyroulias, Georgios A.
In: Biomolecular NMR Assignments, Vol. 10, No. 2, 01.10.2016, p. 379-383.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - NMR study of Met-1 human Angiogenin
T2 - 1H, 13C, 15N backbone and side-chain resonance assignment
AU - Tsika, Aikaterini C.
AU - Chatzileontiadou, Demetra S.M.
AU - Leonidas, Demetres D.
AU - Spyroulias, Georgios A.
PY - 2016/10/1
Y1 - 2016/10/1
N2 - Here, we report the high yield expression and preliminary structural analysis via solution hetero-nuclear NMR spectroscopy of the recombinant Met-1 human Angiogenin. The analysis reveals a well folded as well as, a monomeric polypeptide. Τhe sequence-specific assignment of its 1H, 15N and 13C resonances at high percentage was obtained. Also, using TALOS+ its secondary structure elements were determined.
AB - Here, we report the high yield expression and preliminary structural analysis via solution hetero-nuclear NMR spectroscopy of the recombinant Met-1 human Angiogenin. The analysis reveals a well folded as well as, a monomeric polypeptide. Τhe sequence-specific assignment of its 1H, 15N and 13C resonances at high percentage was obtained. Also, using TALOS+ its secondary structure elements were determined.
KW - Angiogenesis
KW - Angiogenin
KW - NMR spectroscopy
KW - Recombinant protein expression
KW - RNase superfamily
UR - http://www.scopus.com/inward/record.url?scp=84987653452&partnerID=8YFLogxK
U2 - 10.1007/s12104-016-9704-9
DO - 10.1007/s12104-016-9704-9
M3 - Article
C2 - 27624767
AN - SCOPUS:84987653452
VL - 10
SP - 379
EP - 383
JO - Biomolecular NMR Assignments
JF - Biomolecular NMR Assignments
SN - 1874-2718
IS - 2
ER -