NMR study of Met-1 human Angiogenin: 1H, 13C, 15N backbone and side-chain resonance assignment

Aikaterini C. Tsika; Demetra S.M. Chatzileontiadou; Demetres D. Leonidas; Georgios A. Spyroulias

doi:10.1007/s12104-016-9704-9

NMR study of Met-1 human Angiogenin: ¹H, ¹³C, ¹⁵N backbone and side-chain resonance assignment

Aikaterini C. Tsika, Demetra S.M. Chatzileontiadou, Demetres D. Leonidas, Georgios A. Spyroulias

Research output: Contribution to journal › Article › Research › peer-review

3 Citations (Scopus)

Abstract

Here, we report the high yield expression and preliminary structural analysis via solution hetero-nuclear NMR spectroscopy of the recombinant Met-1 human Angiogenin. The analysis reveals a well folded as well as, a monomeric polypeptide. Τhe sequence-specific assignment of its ¹H, ¹⁵N and ¹³C resonances at high percentage was obtained. Also, using TALOS+ its secondary structure elements were determined.

Original language	English
Pages (from-to)	379-383
Number of pages	5
Journal	Biomolecular NMR Assignments
Volume	10
Issue number	2
DOIs	https://doi.org/10.1007/s12104-016-9704-9
Publication status	Published - 1 Oct 2016
Externally published	Yes

Keywords

Angiogenesis
Angiogenin
NMR spectroscopy
Recombinant protein expression
RNase superfamily

Access to Document

10.1007/s12104-016-9704-9

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Cite this

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abstract = "Here, we report the high yield expression and preliminary structural analysis via solution hetero-nuclear NMR spectroscopy of the recombinant Met-1 human Angiogenin. The analysis reveals a well folded as well as, a monomeric polypeptide. Τhe sequence-specific assignment of its 1H, 15N and 13C resonances at high percentage was obtained. Also, using TALOS+ its secondary structure elements were determined.",

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AU - Chatzileontiadou, Demetra S.M.

AU - Leonidas, Demetres D.

AU - Spyroulias, Georgios A.

PY - 2016/10/1

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KW - Angiogenesis

KW - Angiogenin

KW - NMR spectroscopy

KW - Recombinant protein expression

KW - RNase superfamily

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