TY - JOUR
T1 - NMR study of Met-1 human Angiogenin
T2 - 1H, 13C, 15N backbone and side-chain resonance assignment
AU - Tsika, Aikaterini C.
AU - Chatzileontiadou, Demetra S.M.
AU - Leonidas, Demetres D.
AU - Spyroulias, Georgios A.
PY - 2016/10/1
Y1 - 2016/10/1
N2 - Here, we report the high yield expression and preliminary structural analysis via solution hetero-nuclear NMR spectroscopy of the recombinant Met-1 human Angiogenin. The analysis reveals a well folded as well as, a monomeric polypeptide. Τhe sequence-specific assignment of its 1H, 15N and 13C resonances at high percentage was obtained. Also, using TALOS+ its secondary structure elements were determined.
AB - Here, we report the high yield expression and preliminary structural analysis via solution hetero-nuclear NMR spectroscopy of the recombinant Met-1 human Angiogenin. The analysis reveals a well folded as well as, a monomeric polypeptide. Τhe sequence-specific assignment of its 1H, 15N and 13C resonances at high percentage was obtained. Also, using TALOS+ its secondary structure elements were determined.
KW - Angiogenesis
KW - Angiogenin
KW - NMR spectroscopy
KW - Recombinant protein expression
KW - RNase superfamily
UR - http://www.scopus.com/inward/record.url?scp=84987653452&partnerID=8YFLogxK
U2 - 10.1007/s12104-016-9704-9
DO - 10.1007/s12104-016-9704-9
M3 - Article
C2 - 27624767
AN - SCOPUS:84987653452
VL - 10
SP - 379
EP - 383
JO - Biomolecular NMR Assignments
JF - Biomolecular NMR Assignments
SN - 1874-2718
IS - 2
ER -