NMR Spectroscopy of Peptides and Proteins: Practical Considerations

Mark G. Hinds, Raymond S. Norton

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Abstract

High resolution nuclear magnetic resonance (NMR) spectroscopy is the only method available for determining the three-dimensional structures of peptides and proteins in solution at atomic resolution. This article deals with a range of practical considerations associated with such studies, including sample preparation, instrumental setup, one- and two-dimensional NMR methods, interpretation of spectral data, and structure calculations.

Original languageEnglish
Pages (from-to)315-331
Number of pages17
JournalApplied Biochemistry and Biotechnology - Part B Molecular Biotechnology
Volume7
Issue number3
Publication statusPublished - 1997
Externally publishedYes

Keywords

  • 3D structure
  • NMR
  • Peptides
  • Proteins
  • Solvent
  • Temperature

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