NMR-profiles of protein solutions

Bill Pedrini, Pedro Serrano, Biswaranjan Mohanty, Michael Geralt, Kurt Wuthrich

Research output: Contribution to journalArticleResearchpeer-review

15 Citations (Scopus)

Abstract

NMR-Profiles are quantitative one-dimensional (1D) presentations of 2D [15N,1H]-correlation spectra used to monitor the quality of protein solutions prior to and during NMR structure determinations and functional studies. In our current use in structural genomics projects, an NMR-Profile is recorded at the outset of a structure determination, using a uniformly 15N-labeled microscale sample of the protein. We thus assess the extent to which polypeptide backbone resonance assignments can be achieved with given NMR techniques, for example, conventional triple resonance experiments or APSY-NMR. With the availability of sequence-specific polypeptide backbone resonance assignments in the course of the structure determination, an Assigned NMR-Profile is generated, which visualizes the variation of the 15N-1H correlation cross peak intensities along the sequence and thus maps the sequence locations of polypeptide segments for which the NMR line shapes are affected by conformational exchange or other processes. The Assigned NMR-Profile provides a guiding reference during later stages of the structure determination, and is of special interest for monitoring the protein during functional studies, where dynamic features may be modulated during physiological processes. ? 2013 Wiley Periodicals, Inc. Biopolymers 99: 825-831, 2013. Copyright ? 2013 Wiley Periodicals, Inc.
Original languageEnglish
Pages (from-to)825 - 831
Number of pages7
JournalBiopolymers
Volume99
Issue number11
DOIs
Publication statusPublished - 2013
Externally publishedYes

Cite this