NKX2-5eGFP/w hESCs for isolation of human cardiac progenitors and cardiomyocytes

David A Elliott, Stefan R Braam, Katerina Koutsis, Elizabeth S Ng, Robert Jenny, Ebba L Lagerqvist, Christine Biben, Tanya Hatzistavrou, Claire E Hirst, Qing Cissy Yu, Rhys JP Skelton, Dorien Ward-van Oostwaard, Sue Mei Lim, Ouda Khammy, Xueling Li, Susan M Hawes, Richard P Davis, Adam L Goulburn, Robert Passier, Owen WJ PrallJohn M Haynes, Colin W Pouton, David M Kaye, Christine L Mummery, Andrew G Elefanty, Edouard Stanley

Research output: Contribution to journalArticleResearchpeer-review

252 Citations (Scopus)

Abstract

NKX2-5 is expressed in the heart throughout life. We targeted eGFP sequences to the NKX2-5 locus of human embryonic stem cells (hESCs); NKX2-5(eGFP/w) hESCs facilitate quantification of cardiac differentiation, purification of hESC-derived committed cardiac progenitor cells (hESC-CPCs) and cardiomyocytes (hESC-CMs) and the standardization of differentiation protocols. We used NKX2-5 eGFP(+) cells to identify VCAM1 and SIRPA as cell-surface markers expressed in cardiac lineages.
Original languageEnglish
Pages (from-to)1037 - 1040
Number of pages4
JournalNature Methods
Volume8
Issue number12
DOIs
Publication statusPublished - 2011

Cite this

Elliott, D. A., Braam, S. R., Koutsis, K., S Ng, E., Jenny, R., Lagerqvist, E. L., Biben, C., Hatzistavrou, T., Hirst, C. E., Yu, Q. C., Skelton, R. JP., Ward-van Oostwaard, D., Lim, S. M., Khammy, O., Li, X., Hawes, S. M., Davis, R. P., Goulburn, A. L., Passier, R., ... Stanley, E. (2011). NKX2-5eGFP/w hESCs for isolation of human cardiac progenitors and cardiomyocytes. Nature Methods, 8(12), 1037 - 1040. https://doi.org/10.1038/nmeth.1740