Nitroxyl (HNO) suppresses vascular Nox2 oxidase activity

Alyson Anne Miller; Kate Maxwell; Sophocles Chrissobolis; Michelle Leanne Bullen; Jacqueline Ku; Travice Michael De Silva; Stavros Selemidis; Elizabeth Urszula Hooker; Grant Raymond Drummond; Christopher Graeme Sobey; Barbara Kathryn Kemp

doi:10.1016/j.freeradbiomed.2013.02.025

Nitroxyl (HNO) suppresses vascular Nox2 oxidase activity

Alyson Anne Miller, Kate Maxwell, Sophocles Chrissobolis, Michelle Leanne Bullen, Jacqueline Ku, Travice Michael De Silva, Stavros Selemidis, Elizabeth Urszula Hooker, Grant Raymond Drummond, Christopher Graeme Sobey, Barbara Kathryn Kemp

Pharmacology

Research output: Contribution to journal › Article › Research › peer-review

25 Citations (Scopus)

Abstract

Nox2 oxidase activity underlies the oxidative stress and vascular dysfunction associated with several vascular-related diseases. We have reported that nitric oxide (NO) decreases reactive oxygen species production by endothelial Nox2. This study tested the hypothesis that nitroxyl (HNO), the redox sibling of NO, also suppresses vascular Nox2 oxidase activity. Specifically, we examined the influence of two well-characterized HNO donors, Angeli s salt and isopropylamine NONOate (IPA/NO), on Nox2-dependent responses to angiotensin II (reactive oxygen species production and vasoconstriction) in mouse cerebral arteries. Angiotensin II (0.1mumol/L)-stimulated superoxide (measured by lucigenin-enhanced chemiluminescence) and hydrogen peroxide (Amplex red fluorescence) levels in cerebral arteries (pooled basilar and middle cerebral (MCA)) from wild-type (WT) mice were 60 lower (P

Original language	English
Pages (from-to)	264 - 271
Number of pages	8
Journal	Free Radical Biology and Medicine
Volume	60
DOIs	https://doi.org/10.1016/j.freeradbiomed.2013.02.025
Publication status	Published - 2013

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title = "Nitroxyl (HNO) suppresses vascular Nox2 oxidase activity",

abstract = "Nox2 oxidase activity underlies the oxidative stress and vascular dysfunction associated with several vascular-related diseases. We have reported that nitric oxide (NO) decreases reactive oxygen species production by endothelial Nox2. This study tested the hypothesis that nitroxyl (HNO), the redox sibling of NO, also suppresses vascular Nox2 oxidase activity. Specifically, we examined the influence of two well-characterized HNO donors, Angeli s salt and isopropylamine NONOate (IPA/NO), on Nox2-dependent responses to angiotensin II (reactive oxygen species production and vasoconstriction) in mouse cerebral arteries. Angiotensin II (0.1mumol/L)-stimulated superoxide (measured by lucigenin-enhanced chemiluminescence) and hydrogen peroxide (Amplex red fluorescence) levels in cerebral arteries (pooled basilar and middle cerebral (MCA)) from wild-type (WT) mice were 60 lower (P",

author = "Miller, {Alyson Anne} and Kate Maxwell and Sophocles Chrissobolis and Bullen, {Michelle Leanne} and Jacqueline Ku and {De Silva}, {Travice Michael} and Stavros Selemidis and Hooker, {Elizabeth Urszula} and Drummond, {Grant Raymond} and Sobey, {Christopher Graeme} and Kemp, {Barbara Kathryn}",

year = "2013",

doi = "10.1016/j.freeradbiomed.2013.02.025",

language = "English",

volume = "60",

pages = "264 -- 271",

journal = "Free Radical Biology and Medicine",

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publisher = "Elsevier",

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T1 - Nitroxyl (HNO) suppresses vascular Nox2 oxidase activity

AU - Miller, Alyson Anne

AU - Maxwell, Kate

AU - Chrissobolis, Sophocles

AU - Bullen, Michelle Leanne

AU - Ku, Jacqueline

AU - De Silva, Travice Michael

AU - Selemidis, Stavros

AU - Hooker, Elizabeth Urszula

AU - Drummond, Grant Raymond

AU - Sobey, Christopher Graeme

AU - Kemp, Barbara Kathryn

PY - 2013

Y1 - 2013

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AB - Nox2 oxidase activity underlies the oxidative stress and vascular dysfunction associated with several vascular-related diseases. We have reported that nitric oxide (NO) decreases reactive oxygen species production by endothelial Nox2. This study tested the hypothesis that nitroxyl (HNO), the redox sibling of NO, also suppresses vascular Nox2 oxidase activity. Specifically, we examined the influence of two well-characterized HNO donors, Angeli s salt and isopropylamine NONOate (IPA/NO), on Nox2-dependent responses to angiotensin II (reactive oxygen species production and vasoconstriction) in mouse cerebral arteries. Angiotensin II (0.1mumol/L)-stimulated superoxide (measured by lucigenin-enhanced chemiluminescence) and hydrogen peroxide (Amplex red fluorescence) levels in cerebral arteries (pooled basilar and middle cerebral (MCA)) from wild-type (WT) mice were 60 lower (P

UR - http://goo.gl/TsZMvJ

U2 - 10.1016/j.freeradbiomed.2013.02.025

DO - 10.1016/j.freeradbiomed.2013.02.025

M3 - Article

SN - 0891-5849

VL - 60

SP - 264

EP - 271

JO - Free Radical Biology and Medicine

JF - Free Radical Biology and Medicine

ER -

Nitroxyl (HNO) suppresses vascular Nox2 oxidase activity

Abstract

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