Nitroxyl (HNO) suppresses vascular Nox2 oxidase activity

Alyson Anne Miller, Kate Maxwell, Sophocles Chrissobolis, Michelle Leanne Bullen, Jacqueline Ku, Travice Michael De Silva, Stavros Selemidis, Elizabeth Urszula Hooker, Grant Raymond Drummond, Christopher Graeme Sobey, Barbara Kathryn Kemp

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22 Citations (Scopus)

Abstract

Nox2 oxidase activity underlies the oxidative stress and vascular dysfunction associated with several vascular-related diseases. We have reported that nitric oxide (NO) decreases reactive oxygen species production by endothelial Nox2. This study tested the hypothesis that nitroxyl (HNO), the redox sibling of NO, also suppresses vascular Nox2 oxidase activity. Specifically, we examined the influence of two well-characterized HNO donors, Angeli s salt and isopropylamine NONOate (IPA/NO), on Nox2-dependent responses to angiotensin II (reactive oxygen species production and vasoconstriction) in mouse cerebral arteries. Angiotensin II (0.1mumol/L)-stimulated superoxide (measured by lucigenin-enhanced chemiluminescence) and hydrogen peroxide (Amplex red fluorescence) levels in cerebral arteries (pooled basilar and middle cerebral (MCA)) from wild-type (WT) mice were 60 lower (P
Original languageEnglish
Pages (from-to)264 - 271
Number of pages8
JournalFree Radical Biology and Medicine
Volume60
DOIs
Publication statusPublished - 2013

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