NEW TOOLS FOR THE LOCALIZATION OF SECOND MESSENGER SYSTEMS

Roger J. Summers, Lynne R. McMartin

Research output: Contribution to journalArticleResearchpeer-review

Abstract

1. Probes available for the localization of components of second messenger systems include G‐protein oligonucleotides which have been used to produce cDNA probes to label G‐protein mRNA by in situ hybridization histochemistry. 2. Enzymes involved in second messenger responses have been labelled with [3H]‐forskolin (Gs‐linked adenylate cyclase), [3H]‐cAMP (cAMP‐dependent protein kinase) and [3H]‐PDBu2 (protein kinase C). 3. [3H]‐Inositol 1,4,5 trisphosphate labels a site on sarcoplasmic reticulum believed to trigger Ca2+ release. 4. These probes allow the comparison of location of receptors and second messengers and the examination of changes in second messenger systems with drug treatment and disease.

Original languageEnglish
Pages (from-to)549-553
Number of pages5
JournalClinical and Experimental Pharmacology and Physiology
Volume16
Issue number6
DOIs
Publication statusPublished - 1 Jan 1989

Keywords

  • adenylate cyclase
  • autoradiography
  • cAMP‐dependent protein kinase
  • G‐proteins
  • in situ hybridization histochemistry
  • phosphoinositol hydrolysis
  • protein kinase C
  • [H]‐cAMP
  • [H]‐forskolin
  • [H]‐inositol trisphosphate
  • [H]‐phorbol ester

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