NEW TOOLS FOR THE LOCALIZATION OF SECOND MESSENGER SYSTEMS

Roger J. Summers; Lynne R. McMartin

doi:10.1111/j.1440-1681.1989.tb01604.x

NEW TOOLS FOR THE LOCALIZATION OF SECOND MESSENGER SYSTEMS

Roger J. Summers, Lynne R. McMartin

Drug Discovery Biology

Research output: Contribution to journal › Article › Research › peer-review

Abstract

1. Probes available for the localization of components of second messenger systems include G‐protein oligonucleotides which have been used to produce cDNA probes to label G‐protein mRNA by in situ hybridization histochemistry. 2. Enzymes involved in second messenger responses have been labelled with [³H]‐forskolin (Gs‐linked adenylate cyclase), [³H]‐cAMP (cAMP‐dependent protein kinase) and [³H]‐PDBu₂ (protein kinase C). 3. [³H]‐Inositol 1,4,5 trisphosphate labels a site on sarcoplasmic reticulum believed to trigger Ca²⁺ release. 4. These probes allow the comparison of location of receptors and second messengers and the examination of changes in second messenger systems with drug treatment and disease.

Original language	English
Pages (from-to)	549-553
Number of pages	5
Journal	Clinical and Experimental Pharmacology and Physiology
Volume	16
Issue number	6
DOIs	https://doi.org/10.1111/j.1440-1681.1989.tb01604.x
Publication status	Published - 1 Jan 1989

Keywords

adenylate cyclase
autoradiography
cAMP‐dependent protein kinase
G‐proteins
in situ hybridization histochemistry
phosphoinositol hydrolysis
protein kinase C
[H]‐cAMP
[H]‐forskolin
[H]‐inositol trisphosphate
[H]‐phorbol ester

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10.1111/j.1440-1681.1989.tb01604.x

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Cite this

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title = "NEW TOOLS FOR THE LOCALIZATION OF SECOND MESSENGER SYSTEMS",

abstract = "1. Probes available for the localization of components of second messenger systems include G‐protein oligonucleotides which have been used to produce cDNA probes to label G‐protein mRNA by in situ hybridization histochemistry. 2. Enzymes involved in second messenger responses have been labelled with [3H]‐forskolin (Gs‐linked adenylate cyclase), [3H]‐cAMP (cAMP‐dependent protein kinase) and [3H]‐PDBu2 (protein kinase C). 3. [3H]‐Inositol 1,4,5 trisphosphate labels a site on sarcoplasmic reticulum believed to trigger Ca2+ release. 4. These probes allow the comparison of location of receptors and second messengers and the examination of changes in second messenger systems with drug treatment and disease.",

keywords = "adenylate cyclase, autoradiography, cAMP‐dependent protein kinase, G‐proteins, in situ hybridization histochemistry, phosphoinositol hydrolysis, protein kinase C, [H]‐cAMP, [H]‐forskolin, [H]‐inositol trisphosphate, [H]‐phorbol ester",

author = "Summers, {Roger J.} and McMartin, {Lynne R.}",

year = "1989",

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AU - McMartin, Lynne R.

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N2 - 1. Probes available for the localization of components of second messenger systems include G‐protein oligonucleotides which have been used to produce cDNA probes to label G‐protein mRNA by in situ hybridization histochemistry. 2. Enzymes involved in second messenger responses have been labelled with [3H]‐forskolin (Gs‐linked adenylate cyclase), [3H]‐cAMP (cAMP‐dependent protein kinase) and [3H]‐PDBu2 (protein kinase C). 3. [3H]‐Inositol 1,4,5 trisphosphate labels a site on sarcoplasmic reticulum believed to trigger Ca2+ release. 4. These probes allow the comparison of location of receptors and second messengers and the examination of changes in second messenger systems with drug treatment and disease.

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KW - autoradiography

KW - cAMP‐dependent protein kinase

KW - G‐proteins

KW - in situ hybridization histochemistry

KW - phosphoinositol hydrolysis

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KW - [H]‐forskolin

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KW - [H]‐phorbol ester

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