New Monoclonal Antibodies to Defined Cell Surface Proteins on Human Pluripotent Stem Cells

Carmel M. O'Brien; Hun S. Chy; Qi Zhou; Shiri Blumenfeld; Jack W. Lambshead; Xiaodong Liu; Joshua Kie; Bianca D. Capaldo; Tung-Liang Chung; Timothy E. Adams; Tram Phan; John D. Bentley; William J. McKinstry; Karen Oliva; Paul J. McMurrick; Yu-Chieh Wang; Fernando J. Rossello; Geoffrey J. Lindeman; Di Chen; Thierry Jarde; Amander T. Clark; Helen E. Abud; Jane E. Visvader; Christian M. Nefzger; Jose M. Polo; Jeanne F. Loring; Andrew L. Laslett

doi:10.1002/stem.2558

New Monoclonal Antibodies to Defined Cell Surface Proteins on Human Pluripotent Stem Cells

Carmel M. O'Brien, Hun S. Chy, Qi Zhou, Shiri Blumenfeld, Jack W. Lambshead, Xiaodong Liu, Joshua Kie, Bianca D. Capaldo, Tung-Liang Chung, Timothy E. Adams, Tram Phan, John D. Bentley, William J. McKinstry, Karen Oliva, Paul J. McMurrick, Yu-Chieh Wang, Fernando J. Rossello, Geoffrey J. Lindeman, Di Chen, Thierry JardeAmander T. Clark, Helen E. Abud, Jane E. Visvader, Christian M. Nefzger, Jose M. Polo, Jeanne F. Loring, Andrew L. Laslett

Research output: Contribution to journal › Article › Research › peer-review

17 Citations (Scopus)

Abstract

The study and application of human pluripotent stem cells (hPSCs) will be enhanced by the availability of well-characterized monoclonal antibodies (mAbs) detecting cell-surface epitopes. Here, we report generation of seven new mAbs that detect cell surface proteins present on live and fixed human ES cells (hESCs) and human iPS cells (hiPSCs), confirming our previous prediction that these proteins were present on the cell surface of hPSCs. The mAbs all show a high correlation with POU5F1 (OCT4) expression and other hPSC surface markers (TRA-160 and SSEA-4) in hPSC cultures and detect rare OCT4 positive cells in differentiated cell cultures. These mAbs are immunoreactive to cell surface protein epitopes on both primed and naive state hPSCs, providing useful research tools to investigate the cellular mechanisms underlying human pluripotency and states of cellular reprogramming. In addition, we report that subsets of the seven new mAbs are also immunoreactive to human bone marrow-derived mesenchymal stem cells (MSCs), normal human breast subsets and both normal and tumorigenic colorectal cell populations. The mAbs reported here should accelerate the investigation of the nature of pluripotency, and enable development of robust cell separation and tracing technologies to enrich or deplete for hPSCs and other human stem and somatic cell types.

Original language	English
Pages (from-to)	626-640
Number of pages	15
Journal	Stem Cells
Volume	35
Issue number	3
DOIs	https://doi.org/10.1002/stem.2558
Publication status	Published - 1 Mar 2017

Keywords

Breast
Cancer
Cell surface markers
Colorectal
Human embryonic stem cells
Human iPS cells
Monoclonal antibodies
Naive
Pluripotency

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1002/stem.2558

29926191-oaFinal published version, 1.01 MB

Cite this

O'Brien, C. M., Chy, H. S., Zhou, Q., Blumenfeld, S., Lambshead, J. W., Liu, X., Kie, J., Capaldo, B. D., Chung, T.-L., Adams, T. E., Phan, T., Bentley, J. D., McKinstry, W. J., Oliva, K., McMurrick, P. J., Wang, Y.-C., Rossello, F. J., Lindeman, G. J., Chen, D., ... Laslett, A. L. (2017). New Monoclonal Antibodies to Defined Cell Surface Proteins on Human Pluripotent Stem Cells. Stem Cells, 35(3), 626-640. https://doi.org/10.1002/stem.2558

@article{a68a888ec45c4444b46941dd74900aa3,

title = "New Monoclonal Antibodies to Defined Cell Surface Proteins on Human Pluripotent Stem Cells",

abstract = "The study and application of human pluripotent stem cells (hPSCs) will be enhanced by the availability of well-characterized monoclonal antibodies (mAbs) detecting cell-surface epitopes. Here, we report generation of seven new mAbs that detect cell surface proteins present on live and fixed human ES cells (hESCs) and human iPS cells (hiPSCs), confirming our previous prediction that these proteins were present on the cell surface of hPSCs. The mAbs all show a high correlation with POU5F1 (OCT4) expression and other hPSC surface markers (TRA-160 and SSEA-4) in hPSC cultures and detect rare OCT4 positive cells in differentiated cell cultures. These mAbs are immunoreactive to cell surface protein epitopes on both primed and naive state hPSCs, providing useful research tools to investigate the cellular mechanisms underlying human pluripotency and states of cellular reprogramming. In addition, we report that subsets of the seven new mAbs are also immunoreactive to human bone marrow-derived mesenchymal stem cells (MSCs), normal human breast subsets and both normal and tumorigenic colorectal cell populations. The mAbs reported here should accelerate the investigation of the nature of pluripotency, and enable development of robust cell separation and tracing technologies to enrich or deplete for hPSCs and other human stem and somatic cell types. ",

keywords = "Breast, Cancer, Cell surface markers, Colorectal, Human embryonic stem cells, Human iPS cells, Monoclonal antibodies, Naive, Pluripotency",

author = "O'Brien, {Carmel M.} and Chy, {Hun S.} and Qi Zhou and Shiri Blumenfeld and Lambshead, {Jack W.} and Xiaodong Liu and Joshua Kie and Capaldo, {Bianca D.} and Tung-Liang Chung and Adams, {Timothy E.} and Tram Phan and Bentley, {John D.} and McKinstry, {William J.} and Karen Oliva and McMurrick, {Paul J.} and Yu-Chieh Wang and Rossello, {Fernando J.} and Lindeman, {Geoffrey J.} and Di Chen and Thierry Jarde and Clark, {Amander T.} and Abud, {Helen E.} and Visvader, {Jane E.} and Nefzger, {Christian M.} and Polo, {Jose M.} and Loring, {Jeanne F.} and Laslett, {Andrew L.}",

year = "2017",

month = mar,

day = "1",

doi = "10.1002/stem.2558",

language = "English",

volume = "35",

pages = "626--640",

journal = "Stem Cells",

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O'Brien, CM, Chy, HS, Zhou, Q, Blumenfeld, S, Lambshead, JW, Liu, X, Kie, J, Capaldo, BD, Chung, T-L, Adams, TE, Phan, T, Bentley, JD, McKinstry, WJ, Oliva, K, McMurrick, PJ, Wang, Y-C, Rossello, FJ, Lindeman, GJ, Chen, D, Jarde, T, Clark, AT, Abud, HE, Visvader, JE, Nefzger, CM, Polo, JM, Loring, JF & Laslett, AL 2017, 'New Monoclonal Antibodies to Defined Cell Surface Proteins on Human Pluripotent Stem Cells', Stem Cells, vol. 35, no. 3, pp. 626-640. https://doi.org/10.1002/stem.2558

TY - JOUR

T1 - New Monoclonal Antibodies to Defined Cell Surface Proteins on Human Pluripotent Stem Cells

AU - O'Brien, Carmel M.

AU - Chy, Hun S.

AU - Zhou, Qi

AU - Blumenfeld, Shiri

AU - Lambshead, Jack W.

AU - Liu, Xiaodong

AU - Kie, Joshua

AU - Capaldo, Bianca D.

AU - Chung, Tung-Liang

AU - Adams, Timothy E.

AU - Phan, Tram

AU - Bentley, John D.

AU - McKinstry, William J.

AU - Oliva, Karen

AU - McMurrick, Paul J.

AU - Wang, Yu-Chieh

AU - Rossello, Fernando J.

AU - Lindeman, Geoffrey J.

AU - Chen, Di

AU - Jarde, Thierry

AU - Clark, Amander T.

AU - Abud, Helen E.

AU - Visvader, Jane E.

AU - Nefzger, Christian M.

AU - Polo, Jose M.

AU - Loring, Jeanne F.

AU - Laslett, Andrew L.

PY - 2017/3/1

Y1 - 2017/3/1

N2 - The study and application of human pluripotent stem cells (hPSCs) will be enhanced by the availability of well-characterized monoclonal antibodies (mAbs) detecting cell-surface epitopes. Here, we report generation of seven new mAbs that detect cell surface proteins present on live and fixed human ES cells (hESCs) and human iPS cells (hiPSCs), confirming our previous prediction that these proteins were present on the cell surface of hPSCs. The mAbs all show a high correlation with POU5F1 (OCT4) expression and other hPSC surface markers (TRA-160 and SSEA-4) in hPSC cultures and detect rare OCT4 positive cells in differentiated cell cultures. These mAbs are immunoreactive to cell surface protein epitopes on both primed and naive state hPSCs, providing useful research tools to investigate the cellular mechanisms underlying human pluripotency and states of cellular reprogramming. In addition, we report that subsets of the seven new mAbs are also immunoreactive to human bone marrow-derived mesenchymal stem cells (MSCs), normal human breast subsets and both normal and tumorigenic colorectal cell populations. The mAbs reported here should accelerate the investigation of the nature of pluripotency, and enable development of robust cell separation and tracing technologies to enrich or deplete for hPSCs and other human stem and somatic cell types.

AB - The study and application of human pluripotent stem cells (hPSCs) will be enhanced by the availability of well-characterized monoclonal antibodies (mAbs) detecting cell-surface epitopes. Here, we report generation of seven new mAbs that detect cell surface proteins present on live and fixed human ES cells (hESCs) and human iPS cells (hiPSCs), confirming our previous prediction that these proteins were present on the cell surface of hPSCs. The mAbs all show a high correlation with POU5F1 (OCT4) expression and other hPSC surface markers (TRA-160 and SSEA-4) in hPSC cultures and detect rare OCT4 positive cells in differentiated cell cultures. These mAbs are immunoreactive to cell surface protein epitopes on both primed and naive state hPSCs, providing useful research tools to investigate the cellular mechanisms underlying human pluripotency and states of cellular reprogramming. In addition, we report that subsets of the seven new mAbs are also immunoreactive to human bone marrow-derived mesenchymal stem cells (MSCs), normal human breast subsets and both normal and tumorigenic colorectal cell populations. The mAbs reported here should accelerate the investigation of the nature of pluripotency, and enable development of robust cell separation and tracing technologies to enrich or deplete for hPSCs and other human stem and somatic cell types.

KW - Breast

KW - Cancer

KW - Cell surface markers

KW - Colorectal

KW - Human embryonic stem cells

KW - Human iPS cells

KW - Monoclonal antibodies

KW - Naive

KW - Pluripotency

UR - http://www.scopus.com/inward/record.url?scp=85016602811&partnerID=8YFLogxK

U2 - 10.1002/stem.2558

DO - 10.1002/stem.2558

M3 - Article

AN - SCOPUS:85016602811

SN - 1066-5099

VL - 35

SP - 626

EP - 640

JO - Stem Cells

JF - Stem Cells

IS - 3

ER -

New Monoclonal Antibodies to Defined Cell Surface Proteins on Human Pluripotent Stem Cells

Abstract

Keywords

UN SDGs

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Antibody Technologies Facility (MATF)

FlowCore

Genome Modification Platform

Cite this

New Monoclonal Antibodies to Defined Cell Surface Proteins on Human Pluripotent Stem Cells

Abstract

Keywords

UN SDGs

Access to Document

Other files and links

Equipment

Antibody Technologies Facility (MATF)

FlowCore

Genome Modification Platform

Cite this