New Monoclonal Antibodies to Defined Cell Surface Proteins on Human Pluripotent Stem Cells

Carmel M. O'Brien, Hun S. Chy, Qi Zhou, Shiri Blumenfeld, Jack W. Lambshead, Xiaodong Liu, Joshua Kie, Bianca D. Capaldo, Tung-Liang Chung, Timothy E. Adams, Tram Phan, John D. Bentley, William J. McKinstry, Karen Oliva, Paul J. McMurrick, Yu-Chieh Wang, Fernando J. Rossello, Geoffrey J. Lindeman, Di Chen, Thierry Jarde & 7 others Amander T. Clark, Helen E. Abud, Jane E. Visvader, Christian M. Nefzger, Jose M. Polo, Jeanne F. Loring, Andrew L. Laslett

Research output: Contribution to journalArticleResearchpeer-review

Abstract

The study and application of human pluripotent stem cells (hPSCs) will be enhanced by the availability of well-characterized monoclonal antibodies (mAbs) detecting cell-surface epitopes. Here, we report generation of seven new mAbs that detect cell surface proteins present on live and fixed human ES cells (hESCs) and human iPS cells (hiPSCs), confirming our previous prediction that these proteins were present on the cell surface of hPSCs. The mAbs all show a high correlation with POU5F1 (OCT4) expression and other hPSC surface markers (TRA-160 and SSEA-4) in hPSC cultures and detect rare OCT4 positive cells in differentiated cell cultures. These mAbs are immunoreactive to cell surface protein epitopes on both primed and naive state hPSCs, providing useful research tools to investigate the cellular mechanisms underlying human pluripotency and states of cellular reprogramming. In addition, we report that subsets of the seven new mAbs are also immunoreactive to human bone marrow-derived mesenchymal stem cells (MSCs), normal human breast subsets and both normal and tumorigenic colorectal cell populations. The mAbs reported here should accelerate the investigation of the nature of pluripotency, and enable development of robust cell separation and tracing technologies to enrich or deplete for hPSCs and other human stem and somatic cell types. 

LanguageEnglish
Pages626-640
Number of pages15
JournalStem Cells
Volume35
Issue number3
DOIs
StatePublished - 1 Mar 2017

Keywords

  • Breast
  • Cancer
  • Cell surface markers
  • Colorectal
  • Human embryonic stem cells
  • Human iPS cells
  • Monoclonal antibodies
  • Naive
  • Pluripotency

Cite this

O'Brien, Carmel M. ; Chy, Hun S. ; Zhou, Qi ; Blumenfeld, Shiri ; Lambshead, Jack W. ; Liu, Xiaodong ; Kie, Joshua ; Capaldo, Bianca D. ; Chung, Tung-Liang ; Adams, Timothy E. ; Phan, Tram ; Bentley, John D. ; McKinstry, William J. ; Oliva, Karen ; McMurrick, Paul J. ; Wang, Yu-Chieh ; Rossello, Fernando J. ; Lindeman, Geoffrey J. ; Chen, Di ; Jarde, Thierry ; Clark, Amander T. ; Abud, Helen E. ; Visvader, Jane E. ; Nefzger, Christian M. ; Polo, Jose M. ; Loring, Jeanne F. ; Laslett, Andrew L./ New Monoclonal Antibodies to Defined Cell Surface Proteins on Human Pluripotent Stem Cells. In: Stem Cells. 2017 ; Vol. 35, No. 3. pp. 626-640
@article{a68a888ec45c4444b46941dd74900aa3,
title = "New Monoclonal Antibodies to Defined Cell Surface Proteins on Human Pluripotent Stem Cells",
abstract = "The study and application of human pluripotent stem cells (hPSCs) will be enhanced by the availability of well-characterized monoclonal antibodies (mAbs) detecting cell-surface epitopes. Here, we report generation of seven new mAbs that detect cell surface proteins present on live and fixed human ES cells (hESCs) and human iPS cells (hiPSCs), confirming our previous prediction that these proteins were present on the cell surface of hPSCs. The mAbs all show a high correlation with POU5F1 (OCT4) expression and other hPSC surface markers (TRA-160 and SSEA-4) in hPSC cultures and detect rare OCT4 positive cells in differentiated cell cultures. These mAbs are immunoreactive to cell surface protein epitopes on both primed and naive state hPSCs, providing useful research tools to investigate the cellular mechanisms underlying human pluripotency and states of cellular reprogramming. In addition, we report that subsets of the seven new mAbs are also immunoreactive to human bone marrow-derived mesenchymal stem cells (MSCs), normal human breast subsets and both normal and tumorigenic colorectal cell populations. The mAbs reported here should accelerate the investigation of the nature of pluripotency, and enable development of robust cell separation and tracing technologies to enrich or deplete for hPSCs and other human stem and somatic cell types. ",
keywords = "Breast, Cancer, Cell surface markers, Colorectal, Human embryonic stem cells, Human iPS cells, Monoclonal antibodies, Naive, Pluripotency",
author = "O'Brien, {Carmel M.} and Chy, {Hun S.} and Qi Zhou and Shiri Blumenfeld and Lambshead, {Jack W.} and Xiaodong Liu and Joshua Kie and Capaldo, {Bianca D.} and Tung-Liang Chung and Adams, {Timothy E.} and Tram Phan and Bentley, {John D.} and McKinstry, {William J.} and Karen Oliva and McMurrick, {Paul J.} and Yu-Chieh Wang and Rossello, {Fernando J.} and Lindeman, {Geoffrey J.} and Di Chen and Thierry Jarde and Clark, {Amander T.} and Abud, {Helen E.} and Visvader, {Jane E.} and Nefzger, {Christian M.} and Polo, {Jose M.} and Loring, {Jeanne F.} and Laslett, {Andrew L.}",
year = "2017",
month = "3",
day = "1",
doi = "10.1002/stem.2558",
language = "English",
volume = "35",
pages = "626--640",
journal = "Stem Cells",
issn = "1066-5099",
publisher = "AlphaMed Press, Inc",
number = "3",

}

O'Brien, CM, Chy, HS, Zhou, Q, Blumenfeld, S, Lambshead, JW, Liu, X, Kie, J, Capaldo, BD, Chung, T-L, Adams, TE, Phan, T, Bentley, JD, McKinstry, WJ, Oliva, K, McMurrick, PJ, Wang, Y-C, Rossello, FJ, Lindeman, GJ, Chen, D, Jarde, T, Clark, AT, Abud, HE, Visvader, JE, Nefzger, CM, Polo, JM, Loring, JF & Laslett, AL 2017, 'New Monoclonal Antibodies to Defined Cell Surface Proteins on Human Pluripotent Stem Cells' Stem Cells, vol. 35, no. 3, pp. 626-640. DOI: 10.1002/stem.2558

New Monoclonal Antibodies to Defined Cell Surface Proteins on Human Pluripotent Stem Cells. / O'Brien, Carmel M.; Chy, Hun S.; Zhou, Qi; Blumenfeld, Shiri; Lambshead, Jack W.; Liu, Xiaodong; Kie, Joshua; Capaldo, Bianca D.; Chung, Tung-Liang; Adams, Timothy E.; Phan, Tram; Bentley, John D.; McKinstry, William J.; Oliva, Karen; McMurrick, Paul J.; Wang, Yu-Chieh; Rossello, Fernando J.; Lindeman, Geoffrey J.; Chen, Di; Jarde, Thierry; Clark, Amander T.; Abud, Helen E.; Visvader, Jane E.; Nefzger, Christian M.; Polo, Jose M.; Loring, Jeanne F.; Laslett, Andrew L.

In: Stem Cells, Vol. 35, No. 3, 01.03.2017, p. 626-640.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - New Monoclonal Antibodies to Defined Cell Surface Proteins on Human Pluripotent Stem Cells

AU - O'Brien,Carmel M.

AU - Chy,Hun S.

AU - Zhou,Qi

AU - Blumenfeld,Shiri

AU - Lambshead,Jack W.

AU - Liu,Xiaodong

AU - Kie,Joshua

AU - Capaldo,Bianca D.

AU - Chung,Tung-Liang

AU - Adams,Timothy E.

AU - Phan,Tram

AU - Bentley,John D.

AU - McKinstry,William J.

AU - Oliva,Karen

AU - McMurrick,Paul J.

AU - Wang,Yu-Chieh

AU - Rossello,Fernando J.

AU - Lindeman,Geoffrey J.

AU - Chen,Di

AU - Jarde,Thierry

AU - Clark,Amander T.

AU - Abud,Helen E.

AU - Visvader,Jane E.

AU - Nefzger,Christian M.

AU - Polo,Jose M.

AU - Loring,Jeanne F.

AU - Laslett,Andrew L.

PY - 2017/3/1

Y1 - 2017/3/1

N2 - The study and application of human pluripotent stem cells (hPSCs) will be enhanced by the availability of well-characterized monoclonal antibodies (mAbs) detecting cell-surface epitopes. Here, we report generation of seven new mAbs that detect cell surface proteins present on live and fixed human ES cells (hESCs) and human iPS cells (hiPSCs), confirming our previous prediction that these proteins were present on the cell surface of hPSCs. The mAbs all show a high correlation with POU5F1 (OCT4) expression and other hPSC surface markers (TRA-160 and SSEA-4) in hPSC cultures and detect rare OCT4 positive cells in differentiated cell cultures. These mAbs are immunoreactive to cell surface protein epitopes on both primed and naive state hPSCs, providing useful research tools to investigate the cellular mechanisms underlying human pluripotency and states of cellular reprogramming. In addition, we report that subsets of the seven new mAbs are also immunoreactive to human bone marrow-derived mesenchymal stem cells (MSCs), normal human breast subsets and both normal and tumorigenic colorectal cell populations. The mAbs reported here should accelerate the investigation of the nature of pluripotency, and enable development of robust cell separation and tracing technologies to enrich or deplete for hPSCs and other human stem and somatic cell types. 

AB - The study and application of human pluripotent stem cells (hPSCs) will be enhanced by the availability of well-characterized monoclonal antibodies (mAbs) detecting cell-surface epitopes. Here, we report generation of seven new mAbs that detect cell surface proteins present on live and fixed human ES cells (hESCs) and human iPS cells (hiPSCs), confirming our previous prediction that these proteins were present on the cell surface of hPSCs. The mAbs all show a high correlation with POU5F1 (OCT4) expression and other hPSC surface markers (TRA-160 and SSEA-4) in hPSC cultures and detect rare OCT4 positive cells in differentiated cell cultures. These mAbs are immunoreactive to cell surface protein epitopes on both primed and naive state hPSCs, providing useful research tools to investigate the cellular mechanisms underlying human pluripotency and states of cellular reprogramming. In addition, we report that subsets of the seven new mAbs are also immunoreactive to human bone marrow-derived mesenchymal stem cells (MSCs), normal human breast subsets and both normal and tumorigenic colorectal cell populations. The mAbs reported here should accelerate the investigation of the nature of pluripotency, and enable development of robust cell separation and tracing technologies to enrich or deplete for hPSCs and other human stem and somatic cell types. 

KW - Breast

KW - Cancer

KW - Cell surface markers

KW - Colorectal

KW - Human embryonic stem cells

KW - Human iPS cells

KW - Monoclonal antibodies

KW - Naive

KW - Pluripotency

UR - http://www.scopus.com/inward/record.url?scp=85016602811&partnerID=8YFLogxK

U2 - 10.1002/stem.2558

DO - 10.1002/stem.2558

M3 - Article

VL - 35

SP - 626

EP - 640

JO - Stem Cells

T2 - Stem Cells

JF - Stem Cells

SN - 1066-5099

IS - 3

ER -

O'Brien CM, Chy HS, Zhou Q, Blumenfeld S, Lambshead JW, Liu X et al. New Monoclonal Antibodies to Defined Cell Surface Proteins on Human Pluripotent Stem Cells. Stem Cells. 2017 Mar 1;35(3):626-640. Available from, DOI: 10.1002/stem.2558