New Monoclonal Antibodies to Defined Cell Surface Proteins on Human Pluripotent Stem Cells

Carmel M. O'Brien, Hun S. Chy, Qi Zhou, Shiri Blumenfeld, Jack W. Lambshead, Xiaodong Liu, Joshua Kie, Bianca D. Capaldo, Tung-Liang Chung, Timothy E. Adams, Tram Phan, John D. Bentley, William J. McKinstry, Karen Oliva, Paul J. McMurrick, Yu-Chieh Wang, Fernando J. Rossello, Geoffrey J. Lindeman, Di Chen, Thierry JardeAmander T. Clark, Helen E. Abud, Jane E. Visvader, Christian M. Nefzger, Jose M. Polo, Jeanne F. Loring, Andrew L. Laslett

Research output: Contribution to journalArticleResearchpeer-review

17 Citations (Scopus)


The study and application of human pluripotent stem cells (hPSCs) will be enhanced by the availability of well-characterized monoclonal antibodies (mAbs) detecting cell-surface epitopes. Here, we report generation of seven new mAbs that detect cell surface proteins present on live and fixed human ES cells (hESCs) and human iPS cells (hiPSCs), confirming our previous prediction that these proteins were present on the cell surface of hPSCs. The mAbs all show a high correlation with POU5F1 (OCT4) expression and other hPSC surface markers (TRA-160 and SSEA-4) in hPSC cultures and detect rare OCT4 positive cells in differentiated cell cultures. These mAbs are immunoreactive to cell surface protein epitopes on both primed and naive state hPSCs, providing useful research tools to investigate the cellular mechanisms underlying human pluripotency and states of cellular reprogramming. In addition, we report that subsets of the seven new mAbs are also immunoreactive to human bone marrow-derived mesenchymal stem cells (MSCs), normal human breast subsets and both normal and tumorigenic colorectal cell populations. The mAbs reported here should accelerate the investigation of the nature of pluripotency, and enable development of robust cell separation and tracing technologies to enrich or deplete for hPSCs and other human stem and somatic cell types. 

Original languageEnglish
Pages (from-to)626-640
Number of pages15
JournalStem Cells
Issue number3
Publication statusPublished - 1 Mar 2017


  • Breast
  • Cancer
  • Cell surface markers
  • Colorectal
  • Human embryonic stem cells
  • Human iPS cells
  • Monoclonal antibodies
  • Naive
  • Pluripotency

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