New insights into the altered adhesive and mechanical properties of red blood cells parasitized by Babesia bovis

Claire L Hutchings, Ang Li, Kate Marie Fernandez, Taryn Fletcher, Louise A Jackson, John B Molloy, Wayne K Jorgensen, Chwee T Lim, Brian Mark Cooke

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Abstract

Sequestration of parasite-infected red blood cells (RBCs) in the microvasculature is an important pathological feature of both bovine babesiosis caused by Babesia bovis and human malaria caused by Plasmodium falciparum. Surprisingly, when compared with malaria, the cellular and molecular mechanisms that underlie this abnormal circulatory behaviour for RBCs infected with B. bovis have been relatively ignored. Here, we present some novel insights into the adhesive and mechanical changes that occur in B. bovis-infected bovine RBCs and compare them with the alterations that occur in human RBCs infected with P. falciparum. After infection with B. bovis, bovine RBCs become rigid and adhere to vascular endothelial cells under conditions of physiologically relevant flow. These alterations are accompanied by the appearance of ridge-like structures on the RBC surface that are analogous, but morphologically and biochemically different, to the knob-like structures on the surface of human RBCs infected with P. falciparum. Importantly, albeit for a limited number of parasite lines examined here, the extent of these cellular and rheological changes appear to be related to parasite virulence. Future investigations to identify the precise molecular composition of ridges and the proteins that mediate adhesion will provide important insight into the pathogenesis of both babesiosis and malaria.
Original languageEnglish
Pages (from-to)1092 - 1105
Number of pages14
JournalMolecular Microbiology
Volume65
Issue number4
Publication statusPublished - 2007

Cite this

Hutchings, C. L., Li, A., Fernandez, K. M., Fletcher, T., Jackson, L. A., Molloy, J. B., Jorgensen, W. K., Lim, C. T., & Cooke, B. M. (2007). New insights into the altered adhesive and mechanical properties of red blood cells parasitized by Babesia bovis. Molecular Microbiology, 65(4), 1092 - 1105.