New developments in nucleic acid hybridization

H. Wolf; M. Haus; U. Leser; S. Modrow; M. Motz; S. Y. Gu; N. Falser; I. Bandlow; W. Richter; R. Pathmanathan

New developments in nucleic acid hybridization

H. Wolf, M. Haus, U. Leser, S. Modrow, M. Motz, S. Y. Gu, N. Falser, I. Bandlow, W. Richter, R. Pathmanathan

Malaysia Jeffrey Cheah Sch of Med & HS

Research output: Contribution to journal › Review Article › Research › peer-review

3 Citations (Scopus)

Abstract

Nucleic acid hybridization is widely used for scientific applications in specialized laboratories. This paper describes hybridization probes that can be prepared with less specialized equipment. A new indirect 'sandwich' hybridization test is described which allows the use of only one universally usable labelled probe for hybridization tests with specificities for various sequences. The use of different labels and hybridization techniques is also discussed and critically compared. For in situ hybridization, the usability of fixed and embedded materials is tested and evaluated.

Original language	English
Pages (from-to)	373-391
Number of pages	19
Journal	IARC Scientific Publications
Issue number	63
Publication status	Published - 1984

Cite this

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title = "New developments in nucleic acid hybridization",

abstract = "Nucleic acid hybridization is widely used for scientific applications in specialized laboratories. This paper describes hybridization probes that can be prepared with less specialized equipment. A new indirect 'sandwich' hybridization test is described which allows the use of only one universally usable labelled probe for hybridization tests with specificities for various sequences. The use of different labels and hybridization techniques is also discussed and critically compared. For in situ hybridization, the usability of fixed and embedded materials is tested and evaluated.",

author = "H. Wolf and M. Haus and U. Leser and S. Modrow and M. Motz and Gu, \{S. Y.\} and N. Falser and I. Bandlow and W. Richter and R. Pathmanathan",

year = "1984",

language = "English",

pages = "373--391",

journal = "IARC Scientific Publications",

issn = "0300-5038",

publisher = "International Agency for Research on Cancer",

number = "63",

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T1 - New developments in nucleic acid hybridization

AU - Wolf, H.

AU - Haus, M.

AU - Leser, U.

AU - Modrow, S.

AU - Motz, M.

AU - Gu, S. Y.

AU - Falser, N.

AU - Bandlow, I.

AU - Richter, W.

AU - Pathmanathan, R.

PY - 1984

Y1 - 1984

N2 - Nucleic acid hybridization is widely used for scientific applications in specialized laboratories. This paper describes hybridization probes that can be prepared with less specialized equipment. A new indirect 'sandwich' hybridization test is described which allows the use of only one universally usable labelled probe for hybridization tests with specificities for various sequences. The use of different labels and hybridization techniques is also discussed and critically compared. For in situ hybridization, the usability of fixed and embedded materials is tested and evaluated.

AB - Nucleic acid hybridization is widely used for scientific applications in specialized laboratories. This paper describes hybridization probes that can be prepared with less specialized equipment. A new indirect 'sandwich' hybridization test is described which allows the use of only one universally usable labelled probe for hybridization tests with specificities for various sequences. The use of different labels and hybridization techniques is also discussed and critically compared. For in situ hybridization, the usability of fixed and embedded materials is tested and evaluated.

UR - https://www.scopus.com/pages/publications/0021703629

M3 - Review Article

C2 - 6100276

AN - SCOPUS:0021703629

SN - 0300-5038

SP - 373

EP - 391

JO - IARC Scientific Publications

JF - IARC Scientific Publications

IS - 63

ER -

New developments in nucleic acid hybridization

Abstract

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