Neutrophils sustain effective CD8(+) T-cell responses in the respiratory tract following influenza infection

Michelle Tate, Andrew Brooks, Patrick Reading, Justine Mintern

Research output: Contribution to journalArticleResearchpeer-review

58 Citations (Scopus)

Abstract

Neutrophils have an important role in early host protection during influenza A virus infection. Their ability to modulate the virus-specific adaptive immune response is less clear. Here, we have used a mouse model to examine the impact of neutrophils on CD8(+) T-cell responses during influenza virus infection. CD8(+) T-cell priming, expansion, migration, cytokine secretion and cytotoxic capacity were investigated in the virus-infected airways and secondary lymphoid organs. To do this, we utilised a Ly6G-specific monoclonal antibody (mAb; 1A8) that specifically depletes neutrophils in vivo. Neutrophil depletion early after infection with influenza virus strain HKx31 (H3N2) did not alter influenza virus-derived antigen presentation or naive CD8(+) T-cell expansion in the secondary lymphoid organs. Trafficking of virus-specific CD8(+) T cells into the infected pulmonary airways was also unaltered. Instead, early neutropenia reduced both the overall magnitude of influenza virus-specific CD8(+) T cells, together with impaired cytokine production and cytotoxic effector function. Therefore, neutrophils are important participants in anti-viral mechanisms that sustain effective CD8(+) T-cell responses in the respiratory tract of influenza virus-infected mice.
Original languageEnglish
Pages (from-to)197 - 205
Number of pages9
JournalImmunology and Cell Biology
Volume90
Issue number2
DOIs
Publication statusPublished - 2012
Externally publishedYes

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