Neutrophils are important in early control of lung infection by Yersinia pestis

Thomas R. Laws; Martin S. Davey; Richard W. Titball; Roman Lukaszewski

doi:10.1016/j.micinf.2010.01.007

Neutrophils are important in early control of lung infection by Yersinia pestis

Thomas R. Laws, Martin S. Davey, Richard W. Titball, Roman Lukaszewski

Biochemistry & Molecular Biology

Research output: Contribution to journal › Article › Research › peer-review

38 Citations (Scopus)

Abstract

In this paper we evaluate the role of neutrophils in pneumonic plague. Splenic neutrophils from naïve BALB/c mice were found to reduce numbers of culturable Yersinia pestis strain GB in suspension. A murine, BALB/c, intranasal model of pneumonic plague was used in conjunction with in vivo neutrophil ablation, using the GR-1 antibody. This treatment reduced neutrophil numbers without affecting other leukocyte numbers. Neutrophil ablated mice exhibited increased bacterial colonisation of the lung 24 h post infection. Furthermore, exposure of Y. pestis to human neutrophils resulted in a 5-fold reduction in the number of viable bacterial cells, whereas, PBMCs had no effect. Crown

Original language	English
Pages (from-to)	331-335
Number of pages	5
Journal	Microbes and Infection
Volume	12
Issue number	4
DOIs	https://doi.org/10.1016/j.micinf.2010.01.007
Publication status	Published - 1 Apr 2010

Keywords

Ablation
ex vivo
Innate immunity
Intranasal
Neutrophil
Yersinia pestis

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.micinf.2010.01.007

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title = "Neutrophils are important in early control of lung infection by Yersinia pestis",

abstract = "In this paper we evaluate the role of neutrophils in pneumonic plague. Splenic neutrophils from na{\"i}ve BALB/c mice were found to reduce numbers of culturable Yersinia pestis strain GB in suspension. A murine, BALB/c, intranasal model of pneumonic plague was used in conjunction with in vivo neutrophil ablation, using the GR-1 antibody. This treatment reduced neutrophil numbers without affecting other leukocyte numbers. Neutrophil ablated mice exhibited increased bacterial colonisation of the lung 24 h post infection. Furthermore, exposure of Y. pestis to human neutrophils resulted in a 5-fold reduction in the number of viable bacterial cells, whereas, PBMCs had no effect. Crown",

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T1 - Neutrophils are important in early control of lung infection by Yersinia pestis

AU - Laws, Thomas R.

AU - Davey, Martin S.

AU - Titball, Richard W.

AU - Lukaszewski, Roman

PY - 2010/4/1

Y1 - 2010/4/1

N2 - In this paper we evaluate the role of neutrophils in pneumonic plague. Splenic neutrophils from naïve BALB/c mice were found to reduce numbers of culturable Yersinia pestis strain GB in suspension. A murine, BALB/c, intranasal model of pneumonic plague was used in conjunction with in vivo neutrophil ablation, using the GR-1 antibody. This treatment reduced neutrophil numbers without affecting other leukocyte numbers. Neutrophil ablated mice exhibited increased bacterial colonisation of the lung 24 h post infection. Furthermore, exposure of Y. pestis to human neutrophils resulted in a 5-fold reduction in the number of viable bacterial cells, whereas, PBMCs had no effect. Crown

AB - In this paper we evaluate the role of neutrophils in pneumonic plague. Splenic neutrophils from naïve BALB/c mice were found to reduce numbers of culturable Yersinia pestis strain GB in suspension. A murine, BALB/c, intranasal model of pneumonic plague was used in conjunction with in vivo neutrophil ablation, using the GR-1 antibody. This treatment reduced neutrophil numbers without affecting other leukocyte numbers. Neutrophil ablated mice exhibited increased bacterial colonisation of the lung 24 h post infection. Furthermore, exposure of Y. pestis to human neutrophils resulted in a 5-fold reduction in the number of viable bacterial cells, whereas, PBMCs had no effect. Crown

KW - Ablation

KW - ex vivo

KW - Innate immunity

KW - Intranasal

KW - Neutrophil

KW - Yersinia pestis

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M3 - Article

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AN - SCOPUS:77649271965

SN - 1286-4579

VL - 12

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JO - Microbes and Infection

JF - Microbes and Infection

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ER -

Neutrophils are important in early control of lung infection by Yersinia pestis

Abstract

Keywords

UN SDGs

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