Neutrophils are important in early control of lung infection by Yersinia pestis

Thomas R. Laws, Martin S. Davey, Richard W. Titball, Roman Lukaszewski

Research output: Contribution to journalArticleResearchpeer-review

32 Citations (Scopus)

Abstract

In this paper we evaluate the role of neutrophils in pneumonic plague. Splenic neutrophils from naïve BALB/c mice were found to reduce numbers of culturable Yersinia pestis strain GB in suspension. A murine, BALB/c, intranasal model of pneumonic plague was used in conjunction with in vivo neutrophil ablation, using the GR-1 antibody. This treatment reduced neutrophil numbers without affecting other leukocyte numbers. Neutrophil ablated mice exhibited increased bacterial colonisation of the lung 24 h post infection. Furthermore, exposure of Y. pestis to human neutrophils resulted in a 5-fold reduction in the number of viable bacterial cells, whereas, PBMCs had no effect. Crown

Original languageEnglish
Pages (from-to)331-335
Number of pages5
JournalMicrobes and Infection
Volume12
Issue number4
DOIs
Publication statusPublished - 1 Apr 2010

Keywords

  • Ablation
  • ex vivo
  • Innate immunity
  • Intranasal
  • Neutrophil
  • Yersinia pestis

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