TY - JOUR
T1 - Neuroprotective properties of Loranthus parasiticus aqueous fraction against oxidative stress-induced damage in NG108-15 cells
AU - Wong, Daniel Zin Hua
AU - Kadir, Habsah Abdul
AU - Lee, Choy Long
AU - Goh, Bey Hing
N1 - Funding Information:
Acknowledgments We would like to thank University of Malaya for the research funding through Institute of Research and Consultancy (PS238-2008C) and Fundamental Research Grant Scheme (FP009/2010A). We thank Dr. Lee Hong Boon from Cancer Research Initiatives Foundation (CARIF), Sime Darby Medical Centre, Subang Jaya, Selangor, Malaysia for the use of the flow cytometer.
Copyright:
Copyright 2012 Elsevier B.V., All rights reserved.
PY - 2012/7
Y1 - 2012/7
N2 - Loranthus parasiticus, a Chinese folk medicine, has been widely used for the treatment of brain diseases, particularly in southwest China. Hence, the present neuroprotection model was designed to investigate its neuroprotective properties against H 2O 2-induced oxidative stress in NG108-15 cells. L. parasiticus aqueous fraction (LPAF), which was selected in the present study, had proved to be the most active fraction among the other tested extracts and fractions in our previous screening. The restoration of depleted intracellular glutathione (GSH), a major endogenous antioxidant, by LPAF was observed after H 2O 2 insult. Pretreatment with LPAF substantially reduced the production of intracellular reactive oxygen species generated from H 2O 2. Apoptotic features such as externalization of phosphatidylserine and disruption of mitochondrial membrane potential were significantly attenuated by LPAF. In addition, cell cycle analysis revealed a prominent decrease in the H 2O 2-induced sub-G1 population by LPAF. Moreover, apoptotic morphological analysis by DAPI nuclear staining demonstrated that NG108-15 cells treated with H 2O 2 exhibited apoptotic features, while such changes were greatly reduced in cells pretreated with LPAF. Taken together, these findings confirmed that LPAF exerts marked neuroprotective activity, which raises the possibility of potential therapeutic application of LPAF for managing oxidative stress-related neurological disorders and supports the traditional use of L. parasiticus in treating brain-related diseases.
AB - Loranthus parasiticus, a Chinese folk medicine, has been widely used for the treatment of brain diseases, particularly in southwest China. Hence, the present neuroprotection model was designed to investigate its neuroprotective properties against H 2O 2-induced oxidative stress in NG108-15 cells. L. parasiticus aqueous fraction (LPAF), which was selected in the present study, had proved to be the most active fraction among the other tested extracts and fractions in our previous screening. The restoration of depleted intracellular glutathione (GSH), a major endogenous antioxidant, by LPAF was observed after H 2O 2 insult. Pretreatment with LPAF substantially reduced the production of intracellular reactive oxygen species generated from H 2O 2. Apoptotic features such as externalization of phosphatidylserine and disruption of mitochondrial membrane potential were significantly attenuated by LPAF. In addition, cell cycle analysis revealed a prominent decrease in the H 2O 2-induced sub-G1 population by LPAF. Moreover, apoptotic morphological analysis by DAPI nuclear staining demonstrated that NG108-15 cells treated with H 2O 2 exhibited apoptotic features, while such changes were greatly reduced in cells pretreated with LPAF. Taken together, these findings confirmed that LPAF exerts marked neuroprotective activity, which raises the possibility of potential therapeutic application of LPAF for managing oxidative stress-related neurological disorders and supports the traditional use of L. parasiticus in treating brain-related diseases.
KW - Apoptosis
KW - H O
KW - Loranthus parasiticus
KW - Neuroprotection
KW - Oxidative stress
UR - http://www.scopus.com/inward/record.url?scp=84863505303&partnerID=8YFLogxK
U2 - 10.1007/s11418-011-0622-y
DO - 10.1007/s11418-011-0622-y
M3 - Article
C2 - 22318341
AN - SCOPUS:84863505303
SN - 1340-3443
VL - 66
SP - 544
EP - 551
JO - Journal of Natural Medicines
JF - Journal of Natural Medicines
IS - 3
ER -