Neuronal electrophysiological function and control of neurite outgrowth on electrospun polymer nanofibers are cell type dependent

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Abstract

Modeling of cellular environments with nanofabricated biomaterial scaffolds has the potential to improve growth and functional development of cultured cellular models, as well as assist in tissue engineering efforts. An understanding of how such substrates may alter cellular function is critical. Highly plastic central nervous system hippocampal cells and non-network forming peripheral nervous system dorsal root ganglion cells from embryonic rats were cultured upon laminin coated degradable polycaprolactone and non-degradable polystyrene electrospun nanofibrous scaffolds with fiber diameters similar to those of neuronal processes. The two cell types displayed intrinsically different growth patterns on the nanofibrous scaffolds. Hippocampal neurites grew both parallel and perpendicular to the nanofibers, a property that would increase neurite-to-neurite contacts and maximise potential synapse development, essential for extensive network formation in a highly plastic cell type. In contrast, non-network-forming dorsal root ganglion neurons grew neurites exclusively along fibers, recapitulating the simple direct unbranching pathway between sensory ending and synapse in the spinal cord that occurs in vivo. In addition, the two primary neuronal types showed different functional capacities under patch clamp testing. Substrate composition did not alter neuronal functional development, supporting electrospun polycaprolactone and polystyrene as candidate materials for controlled cellular environments in culture and electrospun polycaprolactone for directed neurite outgrowth in tissue engineering applications.
Original languageEnglish
Pages (from-to)1089 - 1095
Number of pages7
JournalTissue Engineering - Part A
Volume20
Issue number5-6
DOIs
Publication statusPublished - 2014

Cite this

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title = "Neuronal electrophysiological function and control of neurite outgrowth on electrospun polymer nanofibers are cell type dependent",
abstract = "Modeling of cellular environments with nanofabricated biomaterial scaffolds has the potential to improve growth and functional development of cultured cellular models, as well as assist in tissue engineering efforts. An understanding of how such substrates may alter cellular function is critical. Highly plastic central nervous system hippocampal cells and non-network forming peripheral nervous system dorsal root ganglion cells from embryonic rats were cultured upon laminin coated degradable polycaprolactone and non-degradable polystyrene electrospun nanofibrous scaffolds with fiber diameters similar to those of neuronal processes. The two cell types displayed intrinsically different growth patterns on the nanofibrous scaffolds. Hippocampal neurites grew both parallel and perpendicular to the nanofibers, a property that would increase neurite-to-neurite contacts and maximise potential synapse development, essential for extensive network formation in a highly plastic cell type. In contrast, non-network-forming dorsal root ganglion neurons grew neurites exclusively along fibers, recapitulating the simple direct unbranching pathway between sensory ending and synapse in the spinal cord that occurs in vivo. In addition, the two primary neuronal types showed different functional capacities under patch clamp testing. Substrate composition did not alter neuronal functional development, supporting electrospun polycaprolactone and polystyrene as candidate materials for controlled cellular environments in culture and electrospun polycaprolactone for directed neurite outgrowth in tissue engineering applications.",
author = "Bourke, {Justin Leigh} and Coleman, {Harold Arthur} and Vi Pham and Forsythe, {John Stanley} and Parkington, {Helena Cecilia}",
year = "2014",
doi = "10.1089/ten.TEA.2013.0295",
language = "English",
volume = "20",
pages = "1089 -- 1095",
journal = "Tissue Engineering - Part A",
issn = "1937-3341",
publisher = "Mary Ann Liebert Inc",
number = "5-6",

}

TY - JOUR

T1 - Neuronal electrophysiological function and control of neurite outgrowth on electrospun polymer nanofibers are cell type dependent

AU - Bourke, Justin Leigh

AU - Coleman, Harold Arthur

AU - Pham, Vi

AU - Forsythe, John Stanley

AU - Parkington, Helena Cecilia

PY - 2014

Y1 - 2014

N2 - Modeling of cellular environments with nanofabricated biomaterial scaffolds has the potential to improve growth and functional development of cultured cellular models, as well as assist in tissue engineering efforts. An understanding of how such substrates may alter cellular function is critical. Highly plastic central nervous system hippocampal cells and non-network forming peripheral nervous system dorsal root ganglion cells from embryonic rats were cultured upon laminin coated degradable polycaprolactone and non-degradable polystyrene electrospun nanofibrous scaffolds with fiber diameters similar to those of neuronal processes. The two cell types displayed intrinsically different growth patterns on the nanofibrous scaffolds. Hippocampal neurites grew both parallel and perpendicular to the nanofibers, a property that would increase neurite-to-neurite contacts and maximise potential synapse development, essential for extensive network formation in a highly plastic cell type. In contrast, non-network-forming dorsal root ganglion neurons grew neurites exclusively along fibers, recapitulating the simple direct unbranching pathway between sensory ending and synapse in the spinal cord that occurs in vivo. In addition, the two primary neuronal types showed different functional capacities under patch clamp testing. Substrate composition did not alter neuronal functional development, supporting electrospun polycaprolactone and polystyrene as candidate materials for controlled cellular environments in culture and electrospun polycaprolactone for directed neurite outgrowth in tissue engineering applications.

AB - Modeling of cellular environments with nanofabricated biomaterial scaffolds has the potential to improve growth and functional development of cultured cellular models, as well as assist in tissue engineering efforts. An understanding of how such substrates may alter cellular function is critical. Highly plastic central nervous system hippocampal cells and non-network forming peripheral nervous system dorsal root ganglion cells from embryonic rats were cultured upon laminin coated degradable polycaprolactone and non-degradable polystyrene electrospun nanofibrous scaffolds with fiber diameters similar to those of neuronal processes. The two cell types displayed intrinsically different growth patterns on the nanofibrous scaffolds. Hippocampal neurites grew both parallel and perpendicular to the nanofibers, a property that would increase neurite-to-neurite contacts and maximise potential synapse development, essential for extensive network formation in a highly plastic cell type. In contrast, non-network-forming dorsal root ganglion neurons grew neurites exclusively along fibers, recapitulating the simple direct unbranching pathway between sensory ending and synapse in the spinal cord that occurs in vivo. In addition, the two primary neuronal types showed different functional capacities under patch clamp testing. Substrate composition did not alter neuronal functional development, supporting electrospun polycaprolactone and polystyrene as candidate materials for controlled cellular environments in culture and electrospun polycaprolactone for directed neurite outgrowth in tissue engineering applications.

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DO - 10.1089/ten.TEA.2013.0295

M3 - Article

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