Abstract
Typically considered to be cell surface sensors of extracellular signals, heterotrimeric GTP-binding protein (G protein)-coupled receptors (GPCRs) control many pathophysiological processes and are the target of 30% of therapeutic drugs. Activated receptors redistribute to endosomes, but researchers have yet to explore whether endosomal receptors generate signals that control complex processes in vivo and are viable therapeutic targets. We report that the substance P (SP) neurokinin 1 receptor (NK1R) signals from endosomes to induce sustained excitation of spinal neurons and pain transmission and that specific antagonism of the NK1R in endosomes with membrane-anchored drug conjugates provides more effective and sustained pain relief than conventional plasma membrane-targeted antagonists. Pharmacological and genetic disruption of clathrin, dynamin, and b-arrestin blocked SP-induced NK1R endocytosis and prevented SP-stimulated activation of cytosolic protein kinase C and nuclear extracellular signal-regulated kinase, as well as transcription. Endocytosis inhibitors prevented sustained SP-induced excitation of neurons in spinal cord slices in vitro and attenuated nociception in vivo. When conjugated to cholestanol to promote endosomal targeting, NK1R antagonists selectively inhibited endosomal signaling and sustained neuronal excitation. Cholestanol conjugation amplified and prolonged the antinociceptive actions of NK1R antagonists. These results reveal a critical role for endosomal signaling of the NK1R in the complex pathophysiology of pain and demonstrate the use of endosomally targeted GPCR antagonists.
| Original language | English |
|---|---|
| Article number | aal3447 |
| Number of pages | 15 |
| Journal | Science Translational Medicine |
| Volume | 9 |
| Issue number | 392 |
| DOIs | |
| Publication status | Published - 31 May 2017 |
Cite this
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Neurokinin 1 receptor signaling in endosomes mediates sustained nociception and is a viable therapeutic target for prolonged pain relief. / Jensen, Dane D; Lieu, TinaMarie; Halls, Michelle L; Veldhuis, Nicholas A; Imlach, Wendy L; Mai, Quynh N; Poole, Daniel P; Quach, Tim; Aurelio, Luigi; Conner, Joshus; Klein Herenbrink, Carmen; Barlow, Nicholas; Simpson, Jamie S; Scanlon, Martin J; Graham, Bimbil; McCluskey, Adam; Robinson, Phillip J; Escriou, Virginie; Nassini, Romina; Materazzi, Serena; Geppetti, Pierangelo; Hicks, Gareth A; Christie, Macdonald J; Porter, Christopher J H; Canals, Meritxell; Bunnett, Nigel W.
In: Science Translational Medicine, Vol. 9, No. 392, aal3447, 31.05.2017.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - Neurokinin 1 receptor signaling in endosomes mediates sustained nociception and is a viable therapeutic target for prolonged pain relief
AU - Jensen, Dane D
AU - Lieu, TinaMarie
AU - Halls, Michelle L
AU - Veldhuis, Nicholas A
AU - Imlach, Wendy L
AU - Mai, Quynh N
AU - Poole, Daniel P
AU - Quach, Tim
AU - Aurelio, Luigi
AU - Conner, Joshus
AU - Klein Herenbrink, Carmen
AU - Barlow, Nicholas
AU - Simpson, Jamie S
AU - Scanlon, Martin J
AU - Graham, Bimbil
AU - McCluskey, Adam
AU - Robinson, Phillip J
AU - Escriou, Virginie
AU - Nassini, Romina
AU - Materazzi, Serena
AU - Geppetti, Pierangelo
AU - Hicks, Gareth A
AU - Christie, Macdonald J
AU - Porter, Christopher J H
AU - Canals, Meritxell
AU - Bunnett, Nigel W
PY - 2017/5/31
Y1 - 2017/5/31
N2 - Typically considered to be cell surface sensors of extracellular signals, heterotrimeric GTP-binding protein (G protein)-coupled receptors (GPCRs) control many pathophysiological processes and are the target of 30% of therapeutic drugs. Activated receptors redistribute to endosomes, but researchers have yet to explore whether endosomal receptors generate signals that control complex processes in vivo and are viable therapeutic targets. We report that the substance P (SP) neurokinin 1 receptor (NK1R) signals from endosomes to induce sustained excitation of spinal neurons and pain transmission and that specific antagonism of the NK1R in endosomes with membrane-anchored drug conjugates provides more effective and sustained pain relief than conventional plasma membrane-targeted antagonists. Pharmacological and genetic disruption of clathrin, dynamin, and b-arrestin blocked SP-induced NK1R endocytosis and prevented SP-stimulated activation of cytosolic protein kinase C and nuclear extracellular signal-regulated kinase, as well as transcription. Endocytosis inhibitors prevented sustained SP-induced excitation of neurons in spinal cord slices in vitro and attenuated nociception in vivo. When conjugated to cholestanol to promote endosomal targeting, NK1R antagonists selectively inhibited endosomal signaling and sustained neuronal excitation. Cholestanol conjugation amplified and prolonged the antinociceptive actions of NK1R antagonists. These results reveal a critical role for endosomal signaling of the NK1R in the complex pathophysiology of pain and demonstrate the use of endosomally targeted GPCR antagonists.
AB - Typically considered to be cell surface sensors of extracellular signals, heterotrimeric GTP-binding protein (G protein)-coupled receptors (GPCRs) control many pathophysiological processes and are the target of 30% of therapeutic drugs. Activated receptors redistribute to endosomes, but researchers have yet to explore whether endosomal receptors generate signals that control complex processes in vivo and are viable therapeutic targets. We report that the substance P (SP) neurokinin 1 receptor (NK1R) signals from endosomes to induce sustained excitation of spinal neurons and pain transmission and that specific antagonism of the NK1R in endosomes with membrane-anchored drug conjugates provides more effective and sustained pain relief than conventional plasma membrane-targeted antagonists. Pharmacological and genetic disruption of clathrin, dynamin, and b-arrestin blocked SP-induced NK1R endocytosis and prevented SP-stimulated activation of cytosolic protein kinase C and nuclear extracellular signal-regulated kinase, as well as transcription. Endocytosis inhibitors prevented sustained SP-induced excitation of neurons in spinal cord slices in vitro and attenuated nociception in vivo. When conjugated to cholestanol to promote endosomal targeting, NK1R antagonists selectively inhibited endosomal signaling and sustained neuronal excitation. Cholestanol conjugation amplified and prolonged the antinociceptive actions of NK1R antagonists. These results reveal a critical role for endosomal signaling of the NK1R in the complex pathophysiology of pain and demonstrate the use of endosomally targeted GPCR antagonists.
UR - http://www.scopus.com/inward/record.url?scp=85020248318&partnerID=8YFLogxK
U2 - 10.1126/scitranslmed.aal3447
DO - 10.1126/scitranslmed.aal3447
M3 - Article
VL - 9
JO - Science Translational Medicine
JF - Science Translational Medicine
SN - 1946-6234
IS - 392
M1 - aal3447
ER -