TY - JOUR
T1 - Nephropathy and elevated BP in mice with podocyte-specific NADPH oxidase 5 expression
AU - Holterman, Chet E.
AU - Thibodeau, Jean-François
AU - Towaij, Chelsea
AU - Gutsol, Alex
AU - Montezano, Augusto C.
AU - Parks, Robin J.
AU - Cooper, Mark E.
AU - Touyz, Rhian M.
AU - Kennedy, Christopher R.J.
PY - 2014/4/1
Y1 - 2014/4/1
N2 - NADPH oxidase (Nox) enzymes are a significant source of reactive oxygen species, which contribute to glomerular podocyte dysfunction. Although studies have implicated Nox1, -2, and -4 in several glomerulopathies, including diabetic nephropathy, little is known regarding the role of Nox5 in this context. We examined Nox5 expression and regulation in kidney biopsies fromdiabetic patients, cultured human podocytes, and a novel mouse model. Nox5 expression increased in human diabetic glomeruli compared with nondiabetic glomeruli. Stimulation with angiotensin II upregulated Nox5 expression in human podocyte cultures and increased reactive oxygen species generation. siRNA-mediated Nox5 knockdown inhibited angiotensin II-stimulated production of reactive oxygen species and altered podocyte cytoskeletal dynamics, resulting in an Rac-mediated motile phenotype. Because the Nox5 gene is absent in rodents, we generated transgenic mice expressing human Nox5 in a podocyte-specific manner (Nox5pod+). Nox5pod+ mice exhibited early onset albuminuria, podocyte foot process effacement, and elevated systolic BP. Subjecting Nox5pod+ mice to streptozotocin-induced diabetes further exacerbated these changes.Our data showthat renalNox5 is upregulated in human diabetic nephropathy andmay alter filtration barrier function and systolic BP through the production of reactive oxygen species. These findings provide the first evidence that podocyte Nox5 has an important role in impaired renal function and hypertension.
AB - NADPH oxidase (Nox) enzymes are a significant source of reactive oxygen species, which contribute to glomerular podocyte dysfunction. Although studies have implicated Nox1, -2, and -4 in several glomerulopathies, including diabetic nephropathy, little is known regarding the role of Nox5 in this context. We examined Nox5 expression and regulation in kidney biopsies fromdiabetic patients, cultured human podocytes, and a novel mouse model. Nox5 expression increased in human diabetic glomeruli compared with nondiabetic glomeruli. Stimulation with angiotensin II upregulated Nox5 expression in human podocyte cultures and increased reactive oxygen species generation. siRNA-mediated Nox5 knockdown inhibited angiotensin II-stimulated production of reactive oxygen species and altered podocyte cytoskeletal dynamics, resulting in an Rac-mediated motile phenotype. Because the Nox5 gene is absent in rodents, we generated transgenic mice expressing human Nox5 in a podocyte-specific manner (Nox5pod+). Nox5pod+ mice exhibited early onset albuminuria, podocyte foot process effacement, and elevated systolic BP. Subjecting Nox5pod+ mice to streptozotocin-induced diabetes further exacerbated these changes.Our data showthat renalNox5 is upregulated in human diabetic nephropathy andmay alter filtration barrier function and systolic BP through the production of reactive oxygen species. These findings provide the first evidence that podocyte Nox5 has an important role in impaired renal function and hypertension.
UR - http://www.scopus.com/inward/record.url?scp=84901452658&partnerID=8YFLogxK
U2 - 10.1681/ASN.2013040371
DO - 10.1681/ASN.2013040371
M3 - Article
C2 - 24262797
AN - SCOPUS:84901452658
VL - 25
SP - 784
EP - 797
JO - Journal of the American Society of Nephrology
JF - Journal of the American Society of Nephrology
SN - 1046-6673
IS - 4
ER -