Neomycin B-cyclen conjugates and their Zn(II) complexes as RNA-binding agents

Bopha Kong, Tanmaya Joshi, Matthew J Belousoff, Yitzhak Tor, Bim Graham, Leone Spiccia

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Three new conjugates featuring the aminoglycoside antibiotic neomycin B linked to the 1,4,7,10-tetraazacyclododecane (cyclen) macrocycle via alkyl chains of varying lengths were synthesized from suitably protected derivatives of these precursors via conventional peptide coupling protocols. The final products were characterized by 1H NMR spectroscopy, mass spectrometry, and elemental analysis. FRET-based measurements examining the ability of the compounds to displace coumarin-labelled kanamycin A or neomycin B from Dy547-labelled prokaryotic ribosomal A-site RNA revealed that they bind to the A-site with slightly higher affinities than the parent aminoglycoside (e.g., IC50 at pH 7 = 1.42–2.30 μM vs. 2.35 μM for neomycin B). This is attributed to the higher overall positive charge of the conjugates, resulting from protonation of the macrocylic amines. Consistent with a predominantly electrostatic mode of interaction, the binding affinities of the conjugates were found to increase with decreasing pH, reflecting a greater degree of protonation at lower pH. The zinc(II) complexes of the neomycin B-cyclen conjugates were found to bind to A-site RNA with even higher affinities (IC50 = 0.85–1.32 μM), due to the Zn(II)-cyclen motif forming coordinative (and/or electrostatic) interactions with the uracil bases and/or phosphate groups within the A-site. These results highlight the potential for the nucleic acid-binding properties of aminoglycosides to be tuned via the covalent attachment of metal complexes, which could ultimately prove useful to the development of new anti-bacterial and anti-viral agents.

Original languageEnglish
Pages (from-to)334-342
Number of pages9
JournalJournal of Inorganic Biochemistry
Volume162
DOIs
Publication statusPublished - 2016

Keywords

  • 1,4,7,10-Tetraazacyclododecane
  • Aminoglycoside derivatives
  • Antibiotics
  • RNA
  • Zinc(II) complexes

Cite this

Kong, Bopha ; Joshi, Tanmaya ; Belousoff, Matthew J ; Tor, Yitzhak ; Graham, Bim ; Spiccia, Leone. / Neomycin B-cyclen conjugates and their Zn(II) complexes as RNA-binding agents. In: Journal of Inorganic Biochemistry. 2016 ; Vol. 162. pp. 334-342.
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abstract = "Three new conjugates featuring the aminoglycoside antibiotic neomycin B linked to the 1,4,7,10-tetraazacyclododecane (cyclen) macrocycle via alkyl chains of varying lengths were synthesized from suitably protected derivatives of these precursors via conventional peptide coupling protocols. The final products were characterized by 1H NMR spectroscopy, mass spectrometry, and elemental analysis. FRET-based measurements examining the ability of the compounds to displace coumarin-labelled kanamycin A or neomycin B from Dy547-labelled prokaryotic ribosomal A-site RNA revealed that they bind to the A-site with slightly higher affinities than the parent aminoglycoside (e.g., IC50 at pH 7 = 1.42–2.30 μM vs. 2.35 μM for neomycin B). This is attributed to the higher overall positive charge of the conjugates, resulting from protonation of the macrocylic amines. Consistent with a predominantly electrostatic mode of interaction, the binding affinities of the conjugates were found to increase with decreasing pH, reflecting a greater degree of protonation at lower pH. The zinc(II) complexes of the neomycin B-cyclen conjugates were found to bind to A-site RNA with even higher affinities (IC50 = 0.85–1.32 μM), due to the Zn(II)-cyclen motif forming coordinative (and/or electrostatic) interactions with the uracil bases and/or phosphate groups within the A-site. These results highlight the potential for the nucleic acid-binding properties of aminoglycosides to be tuned via the covalent attachment of metal complexes, which could ultimately prove useful to the development of new anti-bacterial and anti-viral agents.",
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Neomycin B-cyclen conjugates and their Zn(II) complexes as RNA-binding agents. / Kong, Bopha; Joshi, Tanmaya; Belousoff, Matthew J; Tor, Yitzhak; Graham, Bim; Spiccia, Leone.

In: Journal of Inorganic Biochemistry, Vol. 162, 2016, p. 334-342.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Spiccia, Leone

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