Naturally processed non-canonical HLA-A*02:01 presented peptides

Chopie Hassan, Eric Chabrol, Lorenz Jahn, Michael G D Kester, Arnoud H De Ru, Jan W Drijfhout, Jamie Rossjohn, Frederick J H Falkenburg, Mirjam H M Heemskerk, Stephanie Gras, Peter A Van Veelen

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38 Citations (Scopus)

Abstract

Human leukocyte antigen (HLA) class I molecules generally present peptides (p) of 8 to 11 amino acids (aa) in length. Although an increasing number of examples with lengthy (>11 aa) peptides, presented mostly by HLA-B alleles, have been reported. Here we characterize HLA-A*02:01 restricted, in addition to the HLA-B*0702 and HLA-B*4402 restricted, lengthy peptides (>11 aa) arising from the B-cell ligandome. We analyzed a number of 15-mer peptides presented by HLA-A*02:01, and confirmed pHLA-I formation by HLA folding and thermal stability assays. Surprisingly the binding affinity and stability of the 15-mer epitopes in complex with HLA-A*02:01 were comparable with the values observed for canonical length (8 to 11 aa) HLA-A*02:01-restricted peptides. We solved the structures of two 15-mer epitopes in complex with HLA-A*02:01, within which the peptides adopted distinct super-bulged conformations. Moreover, we demonstrate that T-cells can recognize the 15-mer peptides in the context of HLA-A*02:01, indicating that these 15-mer peptides represent immunogenic ligands. Collectively, our data expand our understanding of longer epitopes in the context of HLA-I, highlighting that they are not limited to the HLA-B family, but can bind the ubiquitous HLA-A*02:01 molecule, and play an important role in T-cell immunity.
Original languageEnglish
Pages (from-to)2593 - 2603
Number of pages11
JournalThe Journal of Biological Chemistry
Volume290
Issue number5
DOIs
Publication statusPublished - 2015

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