Nasal epithelial cells to assess in vitro immune responses to respiratory virus infection in pregnant women with asthma

Rebecca L. Vanders; Alan Hsu; Peter G. Gibson; Vanessa E. Murphy; Peter A.B. Wark

doi:10.1186/s12931-019-1225-5

Nasal epithelial cells to assess in vitro immune responses to respiratory virus infection in pregnant women with asthma

Rebecca L. Vanders
, Alan Hsu
, Peter G. Gibson
, Vanessa E. Murphy
, Peter A.B. Wark

Research output: Contribution to journal › Article › Research › peer-review

14 Citations (Scopus)

Abstract

Respiratory virus-induced asthma exacerbations occur frequently during pregnancy and are associated with adverse outcomes for mother and child. Primary nasal epithelial cells (pNECs) provide a useful method to study immune responses in pregnancy. pNECs were obtained by nasal brushings from pregnant and non-pregnant women with and without asthma. pNECS were infected in vitro with major group Rhinovirus 43 (RV43) and seasonal influenza (H3N2). Following infection, pNECs showed measurable quantities of interferon (IFN)-λ, IL-1β, IL-8, IP-10 and MIP1-α. pNECs provide a safe and effective method for studying respiratory epithelial cell responses during pregnancy.

Original language	English
Article number	259
Number of pages	6
Journal	Respiratory Research
Volume	20
Issue number	1
DOIs	https://doi.org/10.1186/s12931-019-1225-5
Publication status	Published - 20 Nov 2019
Externally published	Yes

Keywords

Asthma
In vitro cultures
Influenza
Nasal cells
Pregnancy
Respiratory infections
Rhinovirus

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10.1186/s12931-019-1225-5Licence: CC BY

Cite this

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abstract = "Respiratory virus-induced asthma exacerbations occur frequently during pregnancy and are associated with adverse outcomes for mother and child. Primary nasal epithelial cells (pNECs) provide a useful method to study immune responses in pregnancy. pNECs were obtained by nasal brushings from pregnant and non-pregnant women with and without asthma. pNECS were infected in vitro with major group Rhinovirus 43 (RV43) and seasonal influenza (H3N2). Following infection, pNECs showed measurable quantities of interferon (IFN)-λ, IL-1β, IL-8, IP-10 and MIP1-α. pNECs provide a safe and effective method for studying respiratory epithelial cell responses during pregnancy.",

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AU - Vanders, Rebecca L.

AU - Hsu, Alan

AU - Gibson, Peter G.

AU - Murphy, Vanessa E.

AU - Wark, Peter A.B.

PY - 2019/11/20

Y1 - 2019/11/20

N2 - Respiratory virus-induced asthma exacerbations occur frequently during pregnancy and are associated with adverse outcomes for mother and child. Primary nasal epithelial cells (pNECs) provide a useful method to study immune responses in pregnancy. pNECs were obtained by nasal brushings from pregnant and non-pregnant women with and without asthma. pNECS were infected in vitro with major group Rhinovirus 43 (RV43) and seasonal influenza (H3N2). Following infection, pNECs showed measurable quantities of interferon (IFN)-λ, IL-1β, IL-8, IP-10 and MIP1-α. pNECs provide a safe and effective method for studying respiratory epithelial cell responses during pregnancy.

AB - Respiratory virus-induced asthma exacerbations occur frequently during pregnancy and are associated with adverse outcomes for mother and child. Primary nasal epithelial cells (pNECs) provide a useful method to study immune responses in pregnancy. pNECs were obtained by nasal brushings from pregnant and non-pregnant women with and without asthma. pNECS were infected in vitro with major group Rhinovirus 43 (RV43) and seasonal influenza (H3N2). Following infection, pNECs showed measurable quantities of interferon (IFN)-λ, IL-1β, IL-8, IP-10 and MIP1-α. pNECs provide a safe and effective method for studying respiratory epithelial cell responses during pregnancy.

KW - Asthma

KW - In vitro cultures

KW - Influenza

KW - Nasal cells

KW - Pregnancy

KW - Respiratory infections

KW - Rhinovirus

UR - https://www.scopus.com/pages/publications/85075475305

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DO - 10.1186/s12931-019-1225-5

M3 - Article

C2 - 31747925

AN - SCOPUS:85075475305

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JO - Respiratory Research

JF - Respiratory Research

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M1 - 259

ER -

Nasal epithelial cells to assess in vitro immune responses to respiratory virus infection in pregnant women with asthma

Abstract

Keywords

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