TY - JOUR
T1 - Nanotubes in nanofibers
T2 - Antibacterial multilayered polylactic acid/halloysite/gentamicin membranes for bone regeneration application
AU - Pierchala, Malgorzata Karolina
AU - Makaremi, Maziyar
AU - Tan, Hui Li
AU - Pushpamalar, Janarthanan
AU - Muniyandy, Saravanan
AU - Solouk, Atefeh
AU - Lee, Sui Mae
AU - Pasbakhsh, Pooria
N1 - Publisher Copyright:
© 2017 Elsevier B.V.
Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.
PY - 2018/8
Y1 - 2018/8
N2 - A multilayered polylactic acid (PLA)/halloysite (HAL) porous membrane encapsulated with aminoglycoside antibiotic (gentamicin) was prepared by electrospinning technique. The prepared electrospun membrane displayed smooth morphology and uniform fiber distribution, while various layers of PLA mats were tightly bounded together. Significant enhancement in mechanical properties and stability was observed in membranes reinforced by HAL and the multilayered membrane loaded with gentamicin, while the drug loaded membrane showed no delamination. In addition, incorporation of HAL led to improvement in thermal stability of the PLA nanofibrous membranes. The in vitro drug release study of gentamicin loaded membrane initially shows a burst release of gentamicin, followed by slow rate of drug release up to 48 h. Antimicrobial efficacy of electrospun membrane was tested on a gram-positive (Staphylococcus aureus) and a gram-negative (Escherichia coli) bacteria, while results indicated that the gentamicin released from electrospun membrane has retained its antibacterial activity. These results signified the potential of multilayered porous PLA/HAL membranes to be utilized in prevention of infection in bone regeneration applications.
AB - A multilayered polylactic acid (PLA)/halloysite (HAL) porous membrane encapsulated with aminoglycoside antibiotic (gentamicin) was prepared by electrospinning technique. The prepared electrospun membrane displayed smooth morphology and uniform fiber distribution, while various layers of PLA mats were tightly bounded together. Significant enhancement in mechanical properties and stability was observed in membranes reinforced by HAL and the multilayered membrane loaded with gentamicin, while the drug loaded membrane showed no delamination. In addition, incorporation of HAL led to improvement in thermal stability of the PLA nanofibrous membranes. The in vitro drug release study of gentamicin loaded membrane initially shows a burst release of gentamicin, followed by slow rate of drug release up to 48 h. Antimicrobial efficacy of electrospun membrane was tested on a gram-positive (Staphylococcus aureus) and a gram-negative (Escherichia coli) bacteria, while results indicated that the gentamicin released from electrospun membrane has retained its antibacterial activity. These results signified the potential of multilayered porous PLA/HAL membranes to be utilized in prevention of infection in bone regeneration applications.
KW - Bone regeneration
KW - Electrospinning
KW - Halloysite nanotubes
KW - Polylactic acid
UR - http://www.scopus.com/inward/record.url?scp=85038611589&partnerID=8YFLogxK
U2 - 10.1016/j.clay.2017.12.016
DO - 10.1016/j.clay.2017.12.016
M3 - Article
AN - SCOPUS:85038611589
VL - 160
SP - 95
EP - 105
JO - Applied Clay Science
JF - Applied Clay Science
SN - 0169-1317
ER -