NanoSIMS analysis of an isotopically labelled organometallic ruthenium(II) drug to probe its distribution and state in vitro

Ronald F.S. Lee; Stéphane Escrig; Marie Croisier; Stéphanie Clerc-Rosset; Graham W. Knott; Anders Meibom; Curt A. Davey; Kai Johnsson; Paul J. Dyson

doi:10.1039/c5cc06983a

NanoSIMS analysis of an isotopically labelled organometallic ruthenium(II) drug to probe its distribution and state in vitro

Ronald F.S. Lee
, Stéphane Escrig
, Marie Croisier
, Stéphanie Clerc-Rosset
, Graham W. Knott
, Anders Meibom
, Curt A. Davey
, Kai Johnsson
, Paul J. Dyson

Research output: Contribution to journal › Article › Research › peer-review

42 Citations (Scopus)

Abstract

The in vitro inter- and intra-cellular distribution of an isotopically labelled ruthenium(ii)-arene (RAPTA) anti-metastatic compound in human ovarian cancer cells was imaged using nano-scale secondary ion mass spectrometry (NanoSIMS). Ultra-high resolution isotopic images of ¹³C, ¹⁵N, and Ru indicate that the phosphine ligand remains coordinated to the ruthenium(ii) ion whereas the arene detaches. The complex localizes mainly on the membrane or at the interface between cells which correlates with its anti-metastatic effects.

Original language	English
Pages (from-to)	16486-16489
Number of pages	4
Journal	Chemical Communications
Volume	51
Issue number	92
DOIs	https://doi.org/10.1039/c5cc06983a
Publication status	Published - 2015
Externally published	Yes

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10.1039/c5cc06983a

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title = "NanoSIMS analysis of an isotopically labelled organometallic ruthenium(II) drug to probe its distribution and state in vitro",

abstract = "The in vitro inter- and intra-cellular distribution of an isotopically labelled ruthenium(ii)-arene (RAPTA) anti-metastatic compound in human ovarian cancer cells was imaged using nano-scale secondary ion mass spectrometry (NanoSIMS). Ultra-high resolution isotopic images of 13C, 15N, and Ru indicate that the phosphine ligand remains coordinated to the ruthenium(ii) ion whereas the arene detaches. The complex localizes mainly on the membrane or at the interface between cells which correlates with its anti-metastatic effects.",

author = "Lee, \{Ronald F.S.\} and St{\'e}phane Escrig and Marie Croisier and St{\'e}phanie Clerc-Rosset and Knott, \{Graham W.\} and Anders Meibom and Davey, \{Curt A.\} and Kai Johnsson and Dyson, \{Paul J.\}",

year = "2015",

doi = "10.1039/c5cc06983a",

language = "English",

volume = "51",

pages = "16486--16489",

journal = "Chemical Communications",

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publisher = "The Royal Society of Chemistry",

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T1 - NanoSIMS analysis of an isotopically labelled organometallic ruthenium(II) drug to probe its distribution and state in vitro

AU - Lee, Ronald F.S.

AU - Escrig, Stéphane

AU - Croisier, Marie

AU - Clerc-Rosset, Stéphanie

AU - Knott, Graham W.

AU - Meibom, Anders

AU - Davey, Curt A.

AU - Johnsson, Kai

AU - Dyson, Paul J.

PY - 2015

Y1 - 2015

N2 - The in vitro inter- and intra-cellular distribution of an isotopically labelled ruthenium(ii)-arene (RAPTA) anti-metastatic compound in human ovarian cancer cells was imaged using nano-scale secondary ion mass spectrometry (NanoSIMS). Ultra-high resolution isotopic images of 13C, 15N, and Ru indicate that the phosphine ligand remains coordinated to the ruthenium(ii) ion whereas the arene detaches. The complex localizes mainly on the membrane or at the interface between cells which correlates with its anti-metastatic effects.

AB - The in vitro inter- and intra-cellular distribution of an isotopically labelled ruthenium(ii)-arene (RAPTA) anti-metastatic compound in human ovarian cancer cells was imaged using nano-scale secondary ion mass spectrometry (NanoSIMS). Ultra-high resolution isotopic images of 13C, 15N, and Ru indicate that the phosphine ligand remains coordinated to the ruthenium(ii) ion whereas the arene detaches. The complex localizes mainly on the membrane or at the interface between cells which correlates with its anti-metastatic effects.

UR - https://www.scopus.com/pages/publications/84946780107

U2 - 10.1039/c5cc06983a

DO - 10.1039/c5cc06983a

M3 - Article

AN - SCOPUS:84946780107

SN - 1359-7345

VL - 51

SP - 16486

EP - 16489

JO - Chemical Communications

JF - Chemical Communications

IS - 92

ER -

NanoSIMS analysis of an isotopically labelled organometallic ruthenium(II) drug to probe its distribution and state in vitro

Abstract

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