NanoSIMS analysis of an isotopically labelled organometallic ruthenium(II) drug to probe its distribution and state in vitro

Ronald F.S. Lee; Stéphane Escrig; Marie Croisier; Stéphanie Clerc-Rosset; Graham W. Knott; Anders Meibom; Curt A. Davey; Kai Johnsson; Paul J. Dyson

doi:10.1039/c5cc06983a

NanoSIMS analysis of an isotopically labelled organometallic ruthenium(II) drug to probe its distribution and state in vitro

Ronald F.S. Lee, Stéphane Escrig, Marie Croisier, Stéphanie Clerc-Rosset, Graham W. Knott, Anders Meibom, Curt A. Davey, Kai Johnsson, Paul J. Dyson

Research output: Contribution to journal › Article › Research › peer-review

39 Citations (Scopus)

Abstract

The in vitro inter- and intra-cellular distribution of an isotopically labelled ruthenium(ii)-arene (RAPTA) anti-metastatic compound in human ovarian cancer cells was imaged using nano-scale secondary ion mass spectrometry (NanoSIMS). Ultra-high resolution isotopic images of ¹³C, ¹⁵N, and Ru indicate that the phosphine ligand remains coordinated to the ruthenium(ii) ion whereas the arene detaches. The complex localizes mainly on the membrane or at the interface between cells which correlates with its anti-metastatic effects.

Original language	English
Pages (from-to)	16486-16489
Number of pages	4
Journal	Chemical Communications
Volume	51
Issue number	92
DOIs	https://doi.org/10.1039/c5cc06983a
Publication status	Published - 2015
Externally published	Yes

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10.1039/c5cc06983a

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abstract = "The in vitro inter- and intra-cellular distribution of an isotopically labelled ruthenium(ii)-arene (RAPTA) anti-metastatic compound in human ovarian cancer cells was imaged using nano-scale secondary ion mass spectrometry (NanoSIMS). Ultra-high resolution isotopic images of 13C, 15N, and Ru indicate that the phosphine ligand remains coordinated to the ruthenium(ii) ion whereas the arene detaches. The complex localizes mainly on the membrane or at the interface between cells which correlates with its anti-metastatic effects.",

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T1 - NanoSIMS analysis of an isotopically labelled organometallic ruthenium(II) drug to probe its distribution and state in vitro

AU - Lee, Ronald F.S.

AU - Escrig, Stéphane

AU - Croisier, Marie

AU - Clerc-Rosset, Stéphanie

AU - Knott, Graham W.

AU - Meibom, Anders

AU - Davey, Curt A.

AU - Johnsson, Kai

AU - Dyson, Paul J.

PY - 2015

Y1 - 2015

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AB - The in vitro inter- and intra-cellular distribution of an isotopically labelled ruthenium(ii)-arene (RAPTA) anti-metastatic compound in human ovarian cancer cells was imaged using nano-scale secondary ion mass spectrometry (NanoSIMS). Ultra-high resolution isotopic images of 13C, 15N, and Ru indicate that the phosphine ligand remains coordinated to the ruthenium(ii) ion whereas the arene detaches. The complex localizes mainly on the membrane or at the interface between cells which correlates with its anti-metastatic effects.

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VL - 51

SP - 16486

EP - 16489

JO - Chemical Communications

JF - Chemical Communications

IS - 92

ER -

NanoSIMS analysis of an isotopically labelled organometallic ruthenium(II) drug to probe its distribution and state in vitro

Abstract

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