NanoSIMS analysis of an isotopically labelled organometallic ruthenium(II) drug to probe its distribution and state in vitro

Ronald F.S. Lee, Stéphane Escrig, Marie Croisier, Stéphanie Clerc-Rosset, Graham W. Knott, Anders Meibom, Curt A. Davey, Kai Johnsson, Paul J. Dyson

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Abstract

The in vitro inter- and intra-cellular distribution of an isotopically labelled ruthenium(ii)-arene (RAPTA) anti-metastatic compound in human ovarian cancer cells was imaged using nano-scale secondary ion mass spectrometry (NanoSIMS). Ultra-high resolution isotopic images of 13C, 15N, and Ru indicate that the phosphine ligand remains coordinated to the ruthenium(ii) ion whereas the arene detaches. The complex localizes mainly on the membrane or at the interface between cells which correlates with its anti-metastatic effects.

Original languageEnglish
Pages (from-to)16486-16489
Number of pages4
JournalChemical Communications
Volume51
Issue number92
DOIs
Publication statusPublished - 2015
Externally publishedYes

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