Nanoscale structure of type I collagen fibrils

Quantitative measurement of D-spacing

Blake Erickson, Ming Fang, Joseph M. Wallace, Bradford G. Orr, Clifford M. Les, Mark M. Banaszak Holl

Research output: Contribution to journalArticleResearchpeer-review

Abstract

This article details a quantitative method to measure the D-periodic spacing of type I collagen fibrils using atomic force microscopy coupled with analysis using a two-dimensional fast fourier transform approach. Instrument calibration, data sampling and data analysis are discussed and comparisons of the data to the complementary methods of electron microscopy and X-ray scattering are made. Examples of the application of this new approach to the analysis of type I collagen morphology in disease models of estrogen depletion and osteogenesis imperfecta (OI) are provided. We demonstrate that it is the D-spacing distribution, not the D-spacing mean, that showed statistically significant differences in estrogen depletion associated with early stage osteoporosis and OI. The ability to quantitatively characterize nanoscale morphological features of type I collagen fibrils will provide important structural information regarding type I collagen in many research areas, including tissue aging and disease, tissue engineering, and gene knockout studies. Furthermore, we also envision potential clinical applications including evaluation of tissue collagen integrity under the impact of diseases or drug treatments.

Original languageEnglish
Pages (from-to)117-126
Number of pages10
JournalBiotechnology Journal
Volume8
Issue number1
DOIs
Publication statusPublished - 1 Jan 2013
Externally publishedYes

Keywords

  • 2D fast fourier transform
  • Atomic force microscopy
  • Collagen fibril
  • D-spacing

Cite this

Erickson, Blake ; Fang, Ming ; Wallace, Joseph M. ; Orr, Bradford G. ; Les, Clifford M. ; Banaszak Holl, Mark M. / Nanoscale structure of type I collagen fibrils : Quantitative measurement of D-spacing. In: Biotechnology Journal. 2013 ; Vol. 8, No. 1. pp. 117-126.
@article{aab256605b0d4261aad741d5fb16ac5d,
title = "Nanoscale structure of type I collagen fibrils: Quantitative measurement of D-spacing",
abstract = "This article details a quantitative method to measure the D-periodic spacing of type I collagen fibrils using atomic force microscopy coupled with analysis using a two-dimensional fast fourier transform approach. Instrument calibration, data sampling and data analysis are discussed and comparisons of the data to the complementary methods of electron microscopy and X-ray scattering are made. Examples of the application of this new approach to the analysis of type I collagen morphology in disease models of estrogen depletion and osteogenesis imperfecta (OI) are provided. We demonstrate that it is the D-spacing distribution, not the D-spacing mean, that showed statistically significant differences in estrogen depletion associated with early stage osteoporosis and OI. The ability to quantitatively characterize nanoscale morphological features of type I collagen fibrils will provide important structural information regarding type I collagen in many research areas, including tissue aging and disease, tissue engineering, and gene knockout studies. Furthermore, we also envision potential clinical applications including evaluation of tissue collagen integrity under the impact of diseases or drug treatments.",
keywords = "2D fast fourier transform, Atomic force microscopy, Collagen fibril, D-spacing",
author = "Blake Erickson and Ming Fang and Wallace, {Joseph M.} and Orr, {Bradford G.} and Les, {Clifford M.} and {Banaszak Holl}, {Mark M.}",
year = "2013",
month = "1",
day = "1",
doi = "10.1002/biot.201200174",
language = "English",
volume = "8",
pages = "117--126",
journal = "Biotechnology Journal",
issn = "1860-6768",
publisher = "Wiley-VCH Verlag GmbH & Co. KGaA",
number = "1",

}

Nanoscale structure of type I collagen fibrils : Quantitative measurement of D-spacing. / Erickson, Blake; Fang, Ming; Wallace, Joseph M.; Orr, Bradford G.; Les, Clifford M.; Banaszak Holl, Mark M.

In: Biotechnology Journal, Vol. 8, No. 1, 01.01.2013, p. 117-126.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Nanoscale structure of type I collagen fibrils

T2 - Quantitative measurement of D-spacing

AU - Erickson, Blake

AU - Fang, Ming

AU - Wallace, Joseph M.

AU - Orr, Bradford G.

AU - Les, Clifford M.

AU - Banaszak Holl, Mark M.

PY - 2013/1/1

Y1 - 2013/1/1

N2 - This article details a quantitative method to measure the D-periodic spacing of type I collagen fibrils using atomic force microscopy coupled with analysis using a two-dimensional fast fourier transform approach. Instrument calibration, data sampling and data analysis are discussed and comparisons of the data to the complementary methods of electron microscopy and X-ray scattering are made. Examples of the application of this new approach to the analysis of type I collagen morphology in disease models of estrogen depletion and osteogenesis imperfecta (OI) are provided. We demonstrate that it is the D-spacing distribution, not the D-spacing mean, that showed statistically significant differences in estrogen depletion associated with early stage osteoporosis and OI. The ability to quantitatively characterize nanoscale morphological features of type I collagen fibrils will provide important structural information regarding type I collagen in many research areas, including tissue aging and disease, tissue engineering, and gene knockout studies. Furthermore, we also envision potential clinical applications including evaluation of tissue collagen integrity under the impact of diseases or drug treatments.

AB - This article details a quantitative method to measure the D-periodic spacing of type I collagen fibrils using atomic force microscopy coupled with analysis using a two-dimensional fast fourier transform approach. Instrument calibration, data sampling and data analysis are discussed and comparisons of the data to the complementary methods of electron microscopy and X-ray scattering are made. Examples of the application of this new approach to the analysis of type I collagen morphology in disease models of estrogen depletion and osteogenesis imperfecta (OI) are provided. We demonstrate that it is the D-spacing distribution, not the D-spacing mean, that showed statistically significant differences in estrogen depletion associated with early stage osteoporosis and OI. The ability to quantitatively characterize nanoscale morphological features of type I collagen fibrils will provide important structural information regarding type I collagen in many research areas, including tissue aging and disease, tissue engineering, and gene knockout studies. Furthermore, we also envision potential clinical applications including evaluation of tissue collagen integrity under the impact of diseases or drug treatments.

KW - 2D fast fourier transform

KW - Atomic force microscopy

KW - Collagen fibril

KW - D-spacing

UR - http://www.scopus.com/inward/record.url?scp=84871749972&partnerID=8YFLogxK

U2 - 10.1002/biot.201200174

DO - 10.1002/biot.201200174

M3 - Article

VL - 8

SP - 117

EP - 126

JO - Biotechnology Journal

JF - Biotechnology Journal

SN - 1860-6768

IS - 1

ER -