Nanocellulose for gel electrophoresis

Llyza Mendoza, Thilina Gunawardhana, Warren Batchelor, Gil Garnier

Research output: Contribution to journalArticleResearchpeer-review

3 Citations (Scopus)

Abstract

Hypothesis: Cellulose nanofibres produced by TEMPO-mediated oxidation can form gels. This study presents a proof-of-concept for gel electrophoresis with nanocellulose (NC). Experiments: TEMPO-oxidised cellulose nanofibre dispersion is chemically cross-linked by inducing amide linkages to produce gel slabs for electrophoretic separation. Nanocellulose gel slabs 1 cm thick containing Tris/Borate/EDTA (TBE) buffer were casted. Different cross-linker types and ratios are investigated to assess the migration of conventional electrophoresis tracking dyes. Findings: Tracking dyes (bromophenol blue and orange G) can diffuse within the gel at different rates and therefore separate. Changing the cross-linker length from EDA to HMDA (C2- to C6-chain) increases the overall network pore size resulting in a faster migration rate for both bromophenol blue and orange G. Increasing the cross-linker concentration stabilises the HMDA-NC gel (no extension) during the electrophoresis run without any effect on the dye migration rate. Increasing the voltage increases the migration rates for both orange G and bromophenol blue. Further development is required to cast the gels evenly and to prevent bubble formation during the cross-linking process. This will enable to effectively separate mixtures of proteins. Nanocellulose gels can become a novel substrate for sustainable biomedical separation and diagnostics by electrophoresis.

Original languageEnglish
Pages (from-to)148-154
Number of pages7
JournalJournal of Colloid and Interface Science
Volume540
DOIs
Publication statusPublished - 22 Mar 2019

Keywords

  • Gel electrophoresis
  • Nanocellulose
  • TEMPO-mediated oxidation

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