N-terminally His-tagged hepatitis B core antigens: construction, expression, purification and antigenicity

Wei Boon Yap, Beng Ti Tey, Michelle Y.T. Ng, Swee Tin Ong, Wen Siang Tan

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13 Citations (Scopus)

Abstract

The core antigen of the hepatitis B virus (HBcAg) has been used widely as a diagnostic reagent for the identification of the viral infection. However, purification using the conventional sucrose density gradient ultracentrifugation is time consuming and costly. To overcome this, HBcAg particles displaying His-tag on their surface were constructed and produced in Escherichia coli. The recombinant His-tagged HBcAgs were purified using immobilized metal affinity chromatography. Transmission electron microscopy and enzyme-linked immunosorbent assay (ELISA) revealed that the displayed His-tag did not impair the formation of the core particles and the antigenicity of HBcAg.

Original languageEnglish
Pages (from-to)125-131
Number of pages7
JournalJournal of Virological Methods
Volume160
Issue number1-2
DOIs
Publication statusPublished - Sep 2009
Externally publishedYes

Keywords

  • Antigenicity
  • Hepatitis B capsid
  • His-tag
  • Protein display
  • Purification

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