N-Terminal T4 Lysozyme Fusion Facilitates Crystallization of a G Protein Coupled Receptor

Yaozhong Zou, William I. Weis, Brian K. Kobilka

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90 Citations (Scopus)

Abstract

A highly crystallizable T4 lysozyme (T4L) was fused to the N-terminus of the β2 adrenergic receptor (β2AR), a G-protein coupled receptor (GPCR) for catecholamines. We demonstrate that the N-terminal fused T4L is sufficiently rigid relative to the receptor to facilitate crystallogenesis without thermostabilizing mutations or the use of a stabilizing antibody, G protein, or protein fused to the 3rd intracellular loop. This approach adds to the protein engineering strategies that enable crystallographic studies of GPCRs alone or in complex with a signaling partner.

Original languageEnglish
Article numbere46039
JournalPLoS ONE
Volume7
Issue number10
DOIs
Publication statusPublished - 4 Oct 2012
Externally publishedYes

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