Myelin protein zero180-199 peptide induced experimental autoimmune neuritis in C57BL/6 mice

David G. Gonsalvez, Sang Won Yoo, Georgina A. Craig, Rhiannon J. Wood, Jessica L. Fletcher, Simon S. Murray, Junhua Xiao

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

5 Citations (Scopus)

Abstract

Mouse models of peripheral demyelinating neuropathy play an important role in enabling the study of disease pathogenesis. Further, induction in transgenic mice allows for the precise interrogation of disease mechanisms, as well as the analysis of the efficacy and mechanisms of potential new therapies. Here we describe a method to successfully induce experimental autoimmune neuritis (EAN) using myelin protein zero (P0)180–199 peptide in combination with Freund’s complete adjuvant and pertussis toxin in the C57BL/6 mouse strain. We also outline a sensitive paradigm of accurately assessing the extent of functional deficits occurring in murine EAN.

Original languageEnglish
Title of host publicationMyelin
Subtitle of host publicationMethods and Protocols
EditorsAshwin Woodhoo
Place of PublicationNew York, NY
PublisherSpringer
Chapter19
Pages243-250
Number of pages8
Edition1st
ISBN (Electronic)9781493978625
ISBN (Print)9781493978618
DOIs
Publication statusPublished - 2018
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1791
ISSN (Print)1064-3745

Keywords

  • C57BL/6 mice
  • Experimental autoimmune neuritis
  • Myelin
  • P0
  • Peripheral neuropathy

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