MX2: a high-flux undulator microfocus beamline serving both the chemical and macromolecular crystallography communities at the Australian Synchrotron

David Aragão; Jun Aishima; Hima Cherukuvada; Robert Clarken; Mark Clift; Nathan Philip Cowieson; Daniel Jesper Ericsson; Christine L. Gee; Sofia Macedo; Nathan Mudie; Santosh Panjikar; Jason Roy Price; Alan Riboldi-Tunnicliffe; Robert Rostan; Rachel Williamson; Thomas Tudor Caradoc-Davies

doi:10.1107/S1600577518003120

MX2: a high-flux undulator microfocus beamline serving both the chemical and macromolecular crystallography communities at the Australian Synchrotron

David Aragão, Jun Aishima, Hima Cherukuvada, Robert Clarken, Mark Clift, Nathan Philip Cowieson, Daniel Jesper Ericsson, Christine L. Gee, Sofia Macedo, Nathan Mudie, Santosh Panjikar, Jason Roy Price, Alan Riboldi-Tunnicliffe, Robert Rostan, Rachel Williamson, Thomas Tudor Caradoc-Davies

Biochemistry & Molecular Biology

Research output: Contribution to journal › Article › Research › peer-review

341 Citations (Scopus)

Abstract

MX2 is an in-vacuum undulator-based crystallography beamline at the 3 GeV Australian Synchrotron. The beamline delivers hard X-rays in the energy range 4.8–21 keV to a focal spot of 22 × 12 µm FWHM (H × V). At 13 keV the flux at the sample is 3.4 × 10¹² photons s⁻¹. The beamline endstation allows robotic handling of cryogenic samples via an updated SSRL SAM robot. This beamline is ideal for weakly diffracting hard-to-crystallize proteins, virus particles, protein assemblies and nucleic acids as well as smaller molecules such as inorganic catalysts and organic drug molecules. The beamline is now mature and has enjoyed a full user program for the last nine years. This paper describes the beamline status, plans for its future and some recent scientific highlights.

Original language	English
Pages (from-to)	885-891
Number of pages	7
Journal	Journal of Synchrotron Radiation
Volume	25
Issue number	3
DOIs	https://doi.org/10.1107/S1600577518003120
Publication status	Published - May 2018

Keywords

anomalous scattering
apertures
collimators
EIGER detector
long wavelengths
macromolecular crystallography
microfocus beamlines
remote access
undulators

Access to Document

10.1107/S1600577518003120

Cite this

Aragão, D., Aishima, J., Cherukuvada, H., Clarken, R., Clift, M., Cowieson, N. P., Ericsson, D. J., Gee, C. L., Macedo, S., Mudie, N., Panjikar, S., Price, J. R., Riboldi-Tunnicliffe, A., Rostan, R., Williamson, R., & Caradoc-Davies, T. T. (2018). MX2: a high-flux undulator microfocus beamline serving both the chemical and macromolecular crystallography communities at the Australian Synchrotron. Journal of Synchrotron Radiation, 25(3), 885-891. https://doi.org/10.1107/S1600577518003120

@article{7d78de35448240a49c969dbfd40c1fbc,

title = "MX2: a high-flux undulator microfocus beamline serving both the chemical and macromolecular crystallography communities at the Australian Synchrotron",

abstract = "MX2 is an in-vacuum undulator-based crystallography beamline at the 3 GeV Australian Synchrotron. The beamline delivers hard X-rays in the energy range 4.8–21 keV to a focal spot of 22 × 12 µm FWHM (H × V). At 13 keV the flux at the sample is 3.4 × 1012 photons s−1. The beamline endstation allows robotic handling of cryogenic samples via an updated SSRL SAM robot. This beamline is ideal for weakly diffracting hard-to-crystallize proteins, virus particles, protein assemblies and nucleic acids as well as smaller molecules such as inorganic catalysts and organic drug molecules. The beamline is now mature and has enjoyed a full user program for the last nine years. This paper describes the beamline status, plans for its future and some recent scientific highlights.",

keywords = "anomalous scattering, apertures, collimators, EIGER detector, long wavelengths, macromolecular crystallography, microfocus beamlines, remote access, undulators",

author = "David Arag{\~a}o and Jun Aishima and Hima Cherukuvada and Robert Clarken and Mark Clift and Cowieson, {Nathan Philip} and Ericsson, {Daniel Jesper} and Gee, {Christine L.} and Sofia Macedo and Nathan Mudie and Santosh Panjikar and Price, {Jason Roy} and Alan Riboldi-Tunnicliffe and Robert Rostan and Rachel Williamson and Caradoc-Davies, {Thomas Tudor}",

year = "2018",

month = may,

doi = "10.1107/S1600577518003120",

language = "English",

volume = "25",

pages = "885--891",

journal = "Journal of Synchrotron Radiation",

issn = "0909-0495",

publisher = "Wiley-Blackwell",

number = "3",

}

Aragão, D, Aishima, J, Cherukuvada, H, Clarken, R, Clift, M, Cowieson, NP, Ericsson, DJ, Gee, CL, Macedo, S, Mudie, N, Panjikar, S, Price, JR, Riboldi-Tunnicliffe, A, Rostan, R, Williamson, R & Caradoc-Davies, TT 2018, 'MX2: a high-flux undulator microfocus beamline serving both the chemical and macromolecular crystallography communities at the Australian Synchrotron', Journal of Synchrotron Radiation, vol. 25, no. 3, pp. 885-891. https://doi.org/10.1107/S1600577518003120

TY - JOUR

T1 - MX2

T2 - a high-flux undulator microfocus beamline serving both the chemical and macromolecular crystallography communities at the Australian Synchrotron

AU - Aragão, David

AU - Aishima, Jun

AU - Cherukuvada, Hima

AU - Clarken, Robert

AU - Clift, Mark

AU - Cowieson, Nathan Philip

AU - Ericsson, Daniel Jesper

AU - Gee, Christine L.

AU - Macedo, Sofia

AU - Mudie, Nathan

AU - Panjikar, Santosh

AU - Price, Jason Roy

AU - Riboldi-Tunnicliffe, Alan

AU - Rostan, Robert

AU - Williamson, Rachel

AU - Caradoc-Davies, Thomas Tudor

PY - 2018/5

Y1 - 2018/5

N2 - MX2 is an in-vacuum undulator-based crystallography beamline at the 3 GeV Australian Synchrotron. The beamline delivers hard X-rays in the energy range 4.8–21 keV to a focal spot of 22 × 12 µm FWHM (H × V). At 13 keV the flux at the sample is 3.4 × 1012 photons s−1. The beamline endstation allows robotic handling of cryogenic samples via an updated SSRL SAM robot. This beamline is ideal for weakly diffracting hard-to-crystallize proteins, virus particles, protein assemblies and nucleic acids as well as smaller molecules such as inorganic catalysts and organic drug molecules. The beamline is now mature and has enjoyed a full user program for the last nine years. This paper describes the beamline status, plans for its future and some recent scientific highlights.

AB - MX2 is an in-vacuum undulator-based crystallography beamline at the 3 GeV Australian Synchrotron. The beamline delivers hard X-rays in the energy range 4.8–21 keV to a focal spot of 22 × 12 µm FWHM (H × V). At 13 keV the flux at the sample is 3.4 × 1012 photons s−1. The beamline endstation allows robotic handling of cryogenic samples via an updated SSRL SAM robot. This beamline is ideal for weakly diffracting hard-to-crystallize proteins, virus particles, protein assemblies and nucleic acids as well as smaller molecules such as inorganic catalysts and organic drug molecules. The beamline is now mature and has enjoyed a full user program for the last nine years. This paper describes the beamline status, plans for its future and some recent scientific highlights.

KW - anomalous scattering

KW - apertures

KW - collimators

KW - EIGER detector

KW - long wavelengths

KW - macromolecular crystallography

KW - microfocus beamlines

KW - remote access

KW - undulators

UR - http://www.scopus.com/inward/record.url?scp=85046355207&partnerID=8YFLogxK

U2 - 10.1107/S1600577518003120

DO - 10.1107/S1600577518003120

M3 - Article

C2 - 29714201

AN - SCOPUS:85046355207

SN - 0909-0495

VL - 25

SP - 885

EP - 891

JO - Journal of Synchrotron Radiation

JF - Journal of Synchrotron Radiation

IS - 3

ER -

MX2: a high-flux undulator microfocus beamline serving both the chemical and macromolecular crystallography communities at the Australian Synchrotron

Abstract

Keywords

Access to Document

Other files and links

ARC Centre of Excellence in Advanced Molecular Imaging

Cite this

MX2: a high-flux undulator microfocus beamline serving both the chemical and macromolecular crystallography communities at the Australian Synchrotron

Abstract

Keywords

Access to Document

Other files and links

Projects

ARC Centre of Excellence in Advanced Molecular Imaging

Cite this