Mutant products of the NF2 tumor suppressor gene are degraded by the ubiquitin-proteasome pathway

Alexis Gautreau, Jan Manent, Bruno Fievet, Daniel Louvard, Marco Giovannini, Monique Arpin

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26 Citations (Scopus)

Abstract

Neurofibromatosis type 2 (NF2), a syndrome associated with multiple tumors of the nervous system, mostly schwannomas, is caused by mutations in the NF2 tumor suppressor gene that encodes schwannomin (Sch). Here we examined NF2 pathogenetic mutations that result in misfolding of the FERM domain. We found that these mutant forms of Sch were efficiently degraded by the ubiquitin-proteasome pathway. In transfected cells, SchδF118 was 3-fold more efficiently degraded than the related molecule ezrin bearing the equivalent mutation. In heterozygous Nf2 knock-out mouse fibroblasts, endogenous mutant Schδ81-121, but not wild type Sch, was also degraded by proteasomes. We further show that this degradation pathway is functional in primary Schwann cells. We analyzed Schδ39-121 expressed in a transgenic mouse model of NF2 and found that Schδ39-121, but not the endogenous wild type Sch, was unstable due to proteasome-mediated degradation. Altogether these results suggest that degradation of mutant Sch mediated by the ubiquitin-proteasome pathway is a physiopathological pathway contributing to the loss of Sch function in NF2 patients.

Original languageEnglish
Pages (from-to)31279-31282
Number of pages4
JournalThe Journal of Biological Chemistry
Volume277
Issue number35
DOIs
Publication statusPublished - 30 Aug 2002
Externally publishedYes

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