Muscarinic receptors in rat uterus

Jocelyn N. Pennefather, Tracey A. Gillman, Frederick Mitchelson

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25 Citations (Scopus)

Abstract

The aim of this study was to characterise the muscarinic receptor present in the uterus of the virgin rat. Homogenate binding studies were undertaken using [3H]quinuclidinyl benzilate as the radioligand and atropine (10 μM) to determine non-specific binding. [3H]Quinuclidinyl benzilate binding was saturable with a Kd of 63 pM and a Bmax of 3 fmol/mg protein. The pKi values obtained using antagonists with high affinity for differing muscarinic receptor subtypes were pirenzepine, 6.2; hexahydrosiladifenidol, 6.9; AF-DX 116 (11-[[2-[(diethylamino)methyl]-1-piperidinyl]-acetyl]5, 11-dihydro-6H-pyrido[2,3-b] [1,4]benzodiazepine-6-one), 7.0; and himbacine, 7.8. These findings suggest that muscarinic M2 receptors are present in rat uterus.

Original languageEnglish
Pages (from-to)297-300
Number of pages4
JournalEuropean Journal of Pharmacology
Volume262
Issue number3
DOIs
Publication statusPublished - 12 Sep 1994

Keywords

  • (Rat)
  • Binding study
  • Muscarinic M receptor
  • Selective antagonist
  • Uterus
  • [H]Quinuclidinyl benzilate

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