Murine helper T lymphocyte response to influenza virus

recognition of haemagglutinin by subtype-specific and cross-reactive T cell clones

Jacqueline M. Katz, Lorena E. Brown, Rosemary A. Ffrench, David O. White

Research output: Contribution to journalArticleResearchpeer-review

4 Citations (Scopus)

Abstract

Helper T cell lines specific for influenza virus were established by continuous culture of Mem 71-Bel (H3) virus-immune spleen cells in the presence of virus and antigen-presenting cells and their specificity assessed in proliferation experiments. At line stimulated in vitro with Mem 71-Bel virus was able to proliferate in response to viruses of the same, and also of different, type A haemagglutinin (HA) subtypes as the immunizing virus but not to a type B influenza virus. A component of this cross-reactivity was due to recognition of the HA molecule. Lines stimulated in vitro with purified disrupted H3 or H2 viruses showed a higher degree of cross-reactivity. Of nine clones isolated from these lines, seven were directed against the HA molecule and recognized the HA1 chain. The HA-specific T cell clones were either subtype-specific, recognizing only viruses of the H3 subtype, or cross-reactive, also recognizing viruses of the H2 subtype of type A (but not type B). Subtype-specific and cross-reactive T cell clones were shown to function as helper T cells in vitro. In addition to collaborating with H3 virus-primed B cells responding to H3 virus in culture, the cross-reactive T cell clone could also provide help for H2 virus-primed B cells making anti-HA antibody in response to H2 virus.

Original languageEnglish
Pages (from-to)257-262
Number of pages6
JournalVaccine
Volume3
Issue number3
DOIs
Publication statusPublished - 1 Jan 1985
Externally publishedYes

Keywords

  • haemagglutinin
  • influenza
  • T lymphocytes
  • Viruses

Cite this

@article{9639cab179fb48cba09ec51c61fa7886,
title = "Murine helper T lymphocyte response to influenza virus: recognition of haemagglutinin by subtype-specific and cross-reactive T cell clones",
abstract = "Helper T cell lines specific for influenza virus were established by continuous culture of Mem 71-Bel (H3) virus-immune spleen cells in the presence of virus and antigen-presenting cells and their specificity assessed in proliferation experiments. At line stimulated in vitro with Mem 71-Bel virus was able to proliferate in response to viruses of the same, and also of different, type A haemagglutinin (HA) subtypes as the immunizing virus but not to a type B influenza virus. A component of this cross-reactivity was due to recognition of the HA molecule. Lines stimulated in vitro with purified disrupted H3 or H2 viruses showed a higher degree of cross-reactivity. Of nine clones isolated from these lines, seven were directed against the HA molecule and recognized the HA1 chain. The HA-specific T cell clones were either subtype-specific, recognizing only viruses of the H3 subtype, or cross-reactive, also recognizing viruses of the H2 subtype of type A (but not type B). Subtype-specific and cross-reactive T cell clones were shown to function as helper T cells in vitro. In addition to collaborating with H3 virus-primed B cells responding to H3 virus in culture, the cross-reactive T cell clone could also provide help for H2 virus-primed B cells making anti-HA antibody in response to H2 virus.",
keywords = "haemagglutinin, influenza, T lymphocytes, Viruses",
author = "Katz, {Jacqueline M.} and Brown, {Lorena E.} and Ffrench, {Rosemary A.} and White, {David O.}",
year = "1985",
month = "1",
day = "1",
doi = "10.1016/0264-410X(85)90118-5",
language = "English",
volume = "3",
pages = "257--262",
journal = "Vaccine",
issn = "0264-410X",
publisher = "Elsevier",
number = "3",

}

Murine helper T lymphocyte response to influenza virus : recognition of haemagglutinin by subtype-specific and cross-reactive T cell clones. / Katz, Jacqueline M.; Brown, Lorena E.; Ffrench, Rosemary A.; White, David O.

In: Vaccine, Vol. 3, No. 3, 01.01.1985, p. 257-262.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Murine helper T lymphocyte response to influenza virus

T2 - recognition of haemagglutinin by subtype-specific and cross-reactive T cell clones

AU - Katz, Jacqueline M.

AU - Brown, Lorena E.

AU - Ffrench, Rosemary A.

AU - White, David O.

PY - 1985/1/1

Y1 - 1985/1/1

N2 - Helper T cell lines specific for influenza virus were established by continuous culture of Mem 71-Bel (H3) virus-immune spleen cells in the presence of virus and antigen-presenting cells and their specificity assessed in proliferation experiments. At line stimulated in vitro with Mem 71-Bel virus was able to proliferate in response to viruses of the same, and also of different, type A haemagglutinin (HA) subtypes as the immunizing virus but not to a type B influenza virus. A component of this cross-reactivity was due to recognition of the HA molecule. Lines stimulated in vitro with purified disrupted H3 or H2 viruses showed a higher degree of cross-reactivity. Of nine clones isolated from these lines, seven were directed against the HA molecule and recognized the HA1 chain. The HA-specific T cell clones were either subtype-specific, recognizing only viruses of the H3 subtype, or cross-reactive, also recognizing viruses of the H2 subtype of type A (but not type B). Subtype-specific and cross-reactive T cell clones were shown to function as helper T cells in vitro. In addition to collaborating with H3 virus-primed B cells responding to H3 virus in culture, the cross-reactive T cell clone could also provide help for H2 virus-primed B cells making anti-HA antibody in response to H2 virus.

AB - Helper T cell lines specific for influenza virus were established by continuous culture of Mem 71-Bel (H3) virus-immune spleen cells in the presence of virus and antigen-presenting cells and their specificity assessed in proliferation experiments. At line stimulated in vitro with Mem 71-Bel virus was able to proliferate in response to viruses of the same, and also of different, type A haemagglutinin (HA) subtypes as the immunizing virus but not to a type B influenza virus. A component of this cross-reactivity was due to recognition of the HA molecule. Lines stimulated in vitro with purified disrupted H3 or H2 viruses showed a higher degree of cross-reactivity. Of nine clones isolated from these lines, seven were directed against the HA molecule and recognized the HA1 chain. The HA-specific T cell clones were either subtype-specific, recognizing only viruses of the H3 subtype, or cross-reactive, also recognizing viruses of the H2 subtype of type A (but not type B). Subtype-specific and cross-reactive T cell clones were shown to function as helper T cells in vitro. In addition to collaborating with H3 virus-primed B cells responding to H3 virus in culture, the cross-reactive T cell clone could also provide help for H2 virus-primed B cells making anti-HA antibody in response to H2 virus.

KW - haemagglutinin

KW - influenza

KW - T lymphocytes

KW - Viruses

UR - http://www.scopus.com/inward/record.url?scp=0022121877&partnerID=8YFLogxK

U2 - 10.1016/0264-410X(85)90118-5

DO - 10.1016/0264-410X(85)90118-5

M3 - Article

VL - 3

SP - 257

EP - 262

JO - Vaccine

JF - Vaccine

SN - 0264-410X

IS - 3

ER -