Murine fecal proteomics: A model system for the detection of potential biomarkers for colorectal cancer

Ching -Seng Ang, Julie Rothacker, Heather Patsiouras, Anthony W Burgess, Edouard Nice

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30 Citations (Scopus)

Abstract

Tumor related products shed into the feces offer a potential source of biomarkers for the detection of colorectal cancer (CRC). Using SDS-PAGE followed by nanoflow reversed-phased LC-MS/MS to analyse fecal samples from Apc(Min/+) mice (that develop spontaneous multiple intestinal neoplasia with age) we have identified 336 proteins (115 proteins of murine origin, 201 from fecal bacteria, 18 associated with food intake and 2 of apparent parasitic origin). 75 of the murine proteins identified in this study are predicted to be extracellular or associated with the cell plasma membrane. Of these proteins, a number of the murine homologues of colorectal cancer associated proteins (CCAP) such as hemoglobin, haptoglobin, hemopexin, alpha-2-macroglobulin and cadherin-17 have been identified, demonstrating the potential of fecal proteomics for detecting potential biomarkers and paving the way for subsequent MS/MS based biomarker studies on similar human samples.
Original languageEnglish
Pages (from-to)3330 - 3340
Number of pages11
JournalJournal of Chromatography A
Volume1217
Issue number19
DOIs
Publication statusPublished - 2010

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