Mumia flava gen. nov., sp. nov., an actinobacterium of the family Nocardioidaceae

Lee Learn Han, Nurullhudda Zainal, Adzzie Shazleen Azman, Nurul-Syakima Ab Mutalib, Kui Hong, Kok Gan Chan

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    31 Citations (Scopus)

    Abstract

    A novel actinobacterial strain, designated MUSC 201T, was isolated from a mangrove soil collected from Kuantan, the capital city of Pahang State in Malaysia. The taxonomic status of this strain was determined using a polyphasic approach. Comparative 16S rRNA gene sequence analysis revealed that strain MUSC 201T represented a novel lineage within the class Actinobacteria. Strain MUSC 201T formed a distinct clade in the family Nocardioidaceae and was most closely related to the members of the genera Nocardioides (16S rRNA gene sequence similarity, 91.9-95.1 ), Aeromicrobium (92.7-94.6 ), Marmoricola (92.5-93.1 ) and Kribbella (91.5-92.4 ). The cells of this strain were irregular coccoid to short rod shaped. The peptidoglycan contained LL-diaminopimelic acid as diagnostic diamino acid and the peptidoglycan type was A3c. The peptidoglycan cell wall contained LL-diaminopimelic acid, glycine, glutamic acid and alanine in a molar ratio of 1.5: 0.9: 1.0: 1.5. The cell-wall sugars were galactose and rhamnose. The predominant menaquinone was MK-9(H4). The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphoglycolipid, glycolipid and four unknown phospholipids. The major cellular fatty acids were C18: 1?9c (30.8 ), C16: 0 (24.1 ), and 10- methyl C18: 0 (13.9 ). The DNA G+C content was 72.0?0.1 mol . On the basis of phylogenetic and phenotypic differences from members of the genera of the family Nocardioidaceae, a novel genus and species, Mumia flava gen. nov., sp. nov. are proposed. The type strain of Mumia flava is MUSC 201T (=DSM 27763T=MCCC 1A00646T=NBRC 109973T).
    Original languageEnglish
    Pages (from-to)1461 - 1467
    Number of pages7
    JournalInternational Journal of Systematic and Evolutionary Microbiology
    Volume64
    Issue numberPart 5
    DOIs
    Publication statusPublished - 2014

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